CAJ | 학술논문

目的探讨RHO激酶在蛛网膜下腔出血(SAH)后患者脑脊液中的表达及其在脑血管痉挛的调控作用.方法采集35例SAH患者脑脊液标本,采集时间为入院后第1、3、7、10、14天,RT-PCR方法检测Rh0激酶mRNA的表达,采用放免法检测脑脊液中内皮素-1(ET-1)浓度,采用TCD检测颅内主要动脉血流速度.结果出血后第3天脑脊液中Rho激酶表达及ET-1浓度增高,在出血后第7天达到高峰,而后逐渐下降.结论 SAH患者脑脊液中Rho激酶的表达与ET-1浓度梯度有关,参与SAH后脑血管痉挛的发生,其含量与病情演变、脑血管痉挛程度有关.
목적 탐토RHO격매재주망막하강출혈(SAH)후환자뇌척액중적표체급기재뇌혈관경련적조공작용.방법 채집35례SAH환자뇌척액표본,채집시간위입원후제1、3、7、10、14천,RT-PCR방법 검측Rh0격매mRNA적표체,채용방면법검측뇌척액중내피소-1(ET-1)농도,채용TCD검측로내주요동맥혈류속도.결과출혈후제3천뇌척액중Rho격매표체급ET-1농도증고,재출혈후제7천체도고봉,이후축점하강.결론 SAH환자뇌척액중Rho격매적표체여ET-1농도제도유관,삼여SAH후뇌혈관경련적발생,기함량여병정연변、뇌혈관경련정도유관.
Objective To investigate the dynamical changes of Rho kinase in the cerebrospinal fluid (CSF) and its relationship with cerebral vascular spasm CVS. Methods CSF were collected on the ist, 3rd, 7th, 10th and 14th day after subarachnoid hemorrhage. The expression of Rho-kinase mRNA in CSF was determined by RT-PCR. The expression of endothelin-1 in CSF was determined by radioimmuno-assay. TCD was used to measure the velocity of the cerebral artery. Results The levels of ET-1 and Rho-kinase mRNA in CSF were re-markably increased on the 3rd day, and reached at the peak on the 7th day after subarachnoid hemorrhage, which were significantly higher than those without CVS. Conclusion There is a positive correlation between the level of Rho-kinase mRNA and ET-1 in CSF. Rho-kinase may participate in the development of CVS.