CAJ | 학술논문

目的研究过氧化物酶体增殖物激活受体α(PPARa)内含子1A/C基因多态性与代谢综合征(MS)的关系.方法选择苏南地区的苏州市区、常熟市及苏北地区的赣榆县队列人群共2348名进行随访调查,排除已知MS病例,实际接受随访1887名.测定腰围、血压、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)、空腹血糖等指标,对新发MS患者进行1:1配对巢式病例-对照研究.使用TaqMan荧光探针法检测PPARα内含子1A/C基因多态性.结果 MS病例组389例,对照组389名.MS病例组的C等位基因频率为22.24%(173/778),低于对照组的24.68%(192/778),但差异无统计学意义(X~2=1.29,P＞0.05).基因型从+AC、CC中MS患者分别占50.70%(363/716)和41.94%(26/62),高血糖患者分别占21.37%(153/716)和11.29%(7/62).与基因型从+AC相比,基因型CC与高血糖之间存在负相关,其调整OR值为0.39(95%CI:0.17～0.90),与MS、腹部肥胖、高TG血症、HDL-C降低及高血压间相关性无统计学意义,其调整OR值分别为0.75(95%CI:0.44～1.28)、0.67(95%CI:0.38～1.17)、0.97(95%CI:0.53～1.76)、0.72(95%CI:0.41～1.25)、0.72(95%CI:0.42～1.25),P值均＞0.05.结论未发现PPARa内含子1A/C的C等位基因与MS相关.相对于基因型从+AC,基因型CC与高血糖之间存在负相关关系.
목적 연구과양화물매체증식물격활수체α(PPARa)내함자1A/C기인다태성여대사종합정(MS)적관계.방법 선택소남지구적소주시구、상숙시급소북지구적공유현대렬인군공2348명진행수방조사,배제이지MS병례,실제접수수방1887명.측정요위、혈압、총담고순(TC)、고밀도지단백담고순(HDL-C)、감유삼지(TG)、공복혈당등지표,대신발MS환자진행1:1배대소식병례-대조연구.사용TaqMan형광탐침법검측PPARα내함자1A/C기인다태성.결과 MS병례조389례,대조조389명.MS병례조적C등위기인빈솔위22.24%(173/778),저우대조조적24.68%(192/778),단차이무통계학의의(X~2=1.29,P＞0.05).기인형종+AC、CC중MS환자분별점50.70%(363/716)화41.94%(26/62),고혈당환자분별점21.37%(153/716)화11.29%(7/62).여기인형종+AC상비,기인형CC여고혈당지간존재부상관,기조정OR치위0.39(95%CI:0.17～0.90),여MS、복부비반、고TG혈증、HDL-C강저급고혈압간상관성무통계학의의,기조정OR치분별위0.75(95%CI:0.44～1.28)、0.67(95%CI:0.38～1.17)、0.97(95%CI:0.53～1.76)、0.72(95%CI:0.41～1.25)、0.72(95%CI:0.42～1.25),P치균＞0.05.결론 미발현PPARa내함자1A/C적C등위기인여MS상관.상대우기인형종+AC,기인형CC여고혈당지간존재부상관관계.
Objective To identify the relationship between genetic polymorphisms of peroxisome proliferator-activated receptor alpha (PPARa) intron 1A/C and metabolic syndrome (MS) in a Chinese population.Methods A population-based case-control study was conducted in Suzhou city,Changshu County and Ganyu County in Jiangsu Province China,on the basis of an ongoing cohort study and 2348 cases were investigated.After the exclusion of the known MS cases,1847 eligible subjects were successfully followed-up and their waist circumference (WC) ,body mass index,blood pressure,total cholesterol (TC) ,high density lipoprotein cholesterol (HDL-C) ,triglycerides (TG) and fasting plasma glucose were measured.Newly diagnosed MS patients were recruited as cases,controls were individual matched with each case.TaqMan fluorescence probe method was used to detect the genetic polymorphism of PPARα intron 1A/C.Results The current analysis consisted of 389 MS patients and 389 matched controls.The C allele gene frequency of PPARα intron 1A/C in the case group was 22.24% (173/778) ,lower than that in the control group,which was 24.68% (192/778) ; whereas the difference was not statistically significant (X~2=1.29,P ＞0.05) .In the genotypes AA+AC and CC,MS patients were accounted for 50.70% (363/716) and 41.94% (26/62) and hyperglycemia accounted for 21.37% (153/716) and 11.29% (7/62) .Compared to the genotypes AA+AC,genotype CC was observed to be inversely associated with hyperglycemia (the adjusted OR=0.39;95% CI:0.17-0.90) but not related to the occurrence of MS (OR=0.75;95% CI:0.44-1.28) and other components of MS e.g.,abdominal obesity (the adjusted OR=0.67 ; 95% CI:0.38-1.17) ,hypertriglyceridemia (the adjusted OR=0.97; 95% CI:0.53-1.76) ,low HDL-C (the adjusted OR =0.72; 95% CI:0.41-1.25) and hypertension (the adjusted OR =0.72; 95% CI:0.42-1.25) all P values ＞ 0.05.Conclusion C allele of PPARα intron 1A/C is not found to be associated with MS in the Chinese population.But comparing with the genotypes AA+AC,there is an inverse association between CC genotype and hyperglycemia.