CAJ | 학술논문

目的探讨Y染色体AZF区域微缺失与生殖激素的关系.方法应用多重PCR扩增对100例无精与严重少精症患者的4个区域15个位点进行AZF基因检测,并采用贝克曼全自动化学发光仪进行生殖激素的测定.采用Epidata建立数据库,应用SAS软件进行均数和方差分析F检验的统计分析.结果 100例患者中发生AZF微缺失的患者13例,检出率为13％.13例AZF微缺失情况:AZFa区缺失1例；AZFb+c+d区缺失4例；AZFc+d区缺失7例；AZFd区缺失1例.Y染色体AZFb+c+d区缺失患者的卵泡刺激素值(40.8±11.3)U/L显著高于无Y染色体缺失患者(16.7±14.3)U/L和AZFa区、AZFc+d区、AZFd区缺失患者(11.8±6.7)U/L,差异具有统计学意义(P＜0.01).结论在无精与严重少精症患者中Y染色体的微缺失以AZFc区和AZFd区缺失最常见,而Y染色体AZFb+c+d区的缺失可能是引起高卵泡刺激素的重要原因之一.
목적 탐토Y염색체AZF구역미결실여생식격소적관계.방법 응용다중PCR확증대100례무정여엄중소정증환자적4개구역15개위점진행AZF기인검측,병채용패극만전자동화학발광의진행생식격소적측정.채용Epidata건립수거고,응용SAS연건진행균수화방차분석F검험적통계분석.결과 100례환자중발생AZF미결실적환자13례,검출솔위13％.13례AZF미결실정황:AZFa구결실1례；AZFb+c+d구결실4례；AZFc+d구결실7례；AZFd구결실1례.Y염색체AZFb+c+d구결실환자적란포자격소치(40.8±11.3)U/L현저고우무Y염색체결실환자(16.7±14.3)U/L화AZFa구、AZFc+d구、AZFd구결실환자(11.8±6.7)U/L,차이구유통계학의의(P＜0.01).결론 재무정여엄중소정증환자중Y염색체적미결실이AZFc구화AZFd구결실최상견,이Y염색체AZFb+c+d구적결실가능시인기고란포자격소적중요원인지일.
Objective To investigate the relationship between follicle stimulating hormone (FSH) and AZF microdeletion on Y chromosome.Methods Fifteen loci of 4 regions of the AZF gene were investigated by multiplex PCR in 100 patients with azoospermia or severe oligozoospermia.The reproductive hormone FSH was detected by access 2 immunoassay system from BECKMAN COULTER.Epidata was set up and analyzed for means.F test of anova was performed.Results The rate of microdeletion was 13％ (13 out of 100 patients).The deletion was on AZFa in 1 patient,AZFb+c+d in 4 patients,AZFc+d in 7 patients,AZFd in 1 patient,respectively.The level of FSH (40.8± 11.3 U/L) in the AZFb+c+d deletion group was significantly higher than that in the group without Y chromosome deletion (16.7± 14.3 U/L) and the other types of deletion (11.8±6.7 U/L) (P＜0.01).Conclusion The common microdeletion regions were AZFc and AZFd on Y chromosome in azoospermia or severe oligozoospermia.The microdeletion of AZFb+c+d was one of the important causes of the high level of FSH.