CAJ | 학술논문

目的构建人基因的重组survivin腺病毒载体,为喉癌的基因治疗研究提供实验基础.方法聚合酶链反应(PCR)克隆survivin基因,PCR产物与克隆载体连接、转化,鉴定阳性克隆测序后再与腺病毒骨架质粒同源重组,对重组克隆行PCR鉴定和Xho Ⅰ、EcoR Ⅰ双酶切鉴定,并包装、纯化.结果核酸序列分析证明,所克隆的survivin基因与文献报道一致,克隆成功,而且外源survivin基因正确地插入到腺病毒载体中,表明已成功构建腺病毒载体pLP-Ad-SUR.结论重组survivin腺病毒载体构建成功.
목적 구건인기인적중조survivin선병독재체,위후암적기인치료연구제공실험기출.방법 취합매련반응(PCR)극륭survivin기인,PCR산물여극륭재체련접、전화,감정양성극륭측서후재여선병독골가질립동원중조,대중조극륭행PCR감정화Xho Ⅰ、EcoR Ⅰ쌍매절감정,병포장、순화.결과 핵산서렬분석증명,소극륭적survivin기인여문헌보도일치,극륭성공,이차외원survivin기인정학지삽입도선병독재체중,표명이성공구건선병독재체pLP-Ad-SUR.결론 중조survivin선병독재체구건성공.
Objective To construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer.Methods The survivin gene was cloned by PCR.After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector.The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus.Results The Sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed.Conclusions A survivin recombinant adenovirus has been successfully constructed.