中华医学超声杂志（电子版）
中華醫學超聲雜誌（電子版）
중화의학초성잡지（전자판）
CHINESE JOURNAL OF MEDICAL ULTRASOUND(ELECTRONICAL VISION)
2008年
2期
223-228
,共6页
朱栋晓%苗宗宁%钱寒光%刘小铭
硃棟曉%苗宗寧%錢寒光%劉小銘
주동효%묘종저%전한광%류소명
超声检查%干细胞%股动脉%血管%再生
超聲檢查%榦細胞%股動脈%血管%再生
초성검사%간세포%고동맥%혈관%재생
Ultrasonography%Stem cells%Femoral artery%Blood vessels%Regeneration
目的 探讨超声在评价兔骨髓间充质干细胞(BMSCs)自体移植促进血管再生过程中的应用价值.方法 把24只新西兰兔分为实验组(12只)和对照组(12只),分离培养实验组兔骨髓间充质干细胞,Brdu标记,建立兔后肢缺血动物模型,将取于自体的骨髓间充质干细胞制成细胞悬液注射于实验组兔缺血部位,对照组注射等量生理盐水.2周后对实验组和对照组兔股动脉及股浅动脉起始段行常规超声及彩色多普勒血流成像(CDFI)检测,检测股动脉血管内径、血流峰值速度及血流加速时间;取缺血部位肌肉组织,行免疫组织化学染色检测实验组移植细胞存在分布情况以及病理学检查血管再生情况.结果 骨髓间充质干细胞移植实验2周后,实验组兔股动脉内径、血流峰值速度均大于对照组,血流加速时间小于对照组 (P＜0.01).免疫组织化学染色结果显示移植部位有移植细胞存在,实验组血管再生情况明显优于对照组.结论 骨髓间充质干细胞移植具有促进血管生成的作用.自体移植有望成为一种简单且有效治疗下肢缺血的方法.高频超声检测股动脉对自体移植效果评价提供了有效实用的方法.
目的 探討超聲在評價兔骨髓間充質榦細胞(BMSCs)自體移植促進血管再生過程中的應用價值.方法 把24隻新西蘭兔分為實驗組(12隻)和對照組(12隻),分離培養實驗組兔骨髓間充質榦細胞,Brdu標記,建立兔後肢缺血動物模型,將取于自體的骨髓間充質榦細胞製成細胞懸液註射于實驗組兔缺血部位,對照組註射等量生理鹽水.2週後對實驗組和對照組兔股動脈及股淺動脈起始段行常規超聲及綵色多普勒血流成像(CDFI)檢測,檢測股動脈血管內徑、血流峰值速度及血流加速時間;取缺血部位肌肉組織,行免疫組織化學染色檢測實驗組移植細胞存在分佈情況以及病理學檢查血管再生情況.結果 骨髓間充質榦細胞移植實驗2週後,實驗組兔股動脈內徑、血流峰值速度均大于對照組,血流加速時間小于對照組 (P＜0.01).免疫組織化學染色結果顯示移植部位有移植細胞存在,實驗組血管再生情況明顯優于對照組.結論 骨髓間充質榦細胞移植具有促進血管生成的作用.自體移植有望成為一種簡單且有效治療下肢缺血的方法.高頻超聲檢測股動脈對自體移植效果評價提供瞭有效實用的方法.
목적 탐토초성재평개토골수간충질간세포(BMSCs)자체이식촉진혈관재생과정중적응용개치.방법 파24지신서란토분위실험조(12지)화대조조(12지),분리배양실험조토골수간충질간세포,Brdu표기,건립토후지결혈동물모형,장취우자체적골수간충질간세포제성세포현액주사우실험조토결혈부위,대조조주사등량생리염수.2주후대실험조화대조조토고동맥급고천동맥기시단행상규초성급채색다보륵혈류성상(CDFI)검측,검측고동맥혈관내경、혈류봉치속도급혈류가속시간;취결혈부위기육조직,행면역조직화학염색검측실험조이식세포존재분포정황이급병이학검사혈관재생정황.결과 골수간충질간세포이식실험2주후,실험조토고동맥내경、혈류봉치속도균대우대조조,혈류가속시간소우대조조 (P＜0.01).면역조직화학염색결과현시이식부위유이식세포존재,실험조혈관재생정황명현우우대조조.결론 골수간충질간세포이식구유촉진혈관생성적작용.자체이식유망성위일충간단차유효치료하지결혈적방법.고빈초성검측고동맥대자체이식효과평개제공료유효실용적방법.
Objective To investigate the significance and effect of ultrasonic diagnosis on the autologous bone marrow mesenchymal stem cells (MSC) in angiogenesis. Methods Twenty-four New Zealand rabbits were divided into experiment group (12) and the control group (12). Then rabbit bone marrow MSCs from experiment group were isolated, caltured and marked with Brdu. After ischemic hind limb animal model on all rabbits was set up, autologous bone marrow MSCs were directly injected into the ischemic hind limb muscles in experiment group while same volume normal saline was used in the control group. Two weeks after the implantation of autologous bone marrow MSCs, 2D and color Doppler flow imaging (CDFI) detection were used in rabbit femoral artery of the two groups to observe the inner diameter of the blood vessel, the peak velocity and the acceleration time. The disposition of transplaned cells and the state of angiogenesis in ischemic muscles were assessed using immunofluorescence staining. Results The results of 2D and Doppler ultrasound detection showed the inner diameter of the blood vessel and the peak velocity of the blood current in experiment group obviously higher than that of the control group , and the acceleration time was obviously smaller than that of the control group P<0.01. The immunofluorescence staining showed there were transplanted cells existed in transplanted portion and state of angiogenesis was supurior obviously than that of the control. Conclusions Bone marrow MSCs had the effect to promote angiogenesis. Implantation of autologous bone marrow MSCs was a simple and efficient therapeutic method for the ischemia hind limb. Using high-frequency ultrasound to detect femoral artery may provide a practical and useful method to evaluate the effect on implanted autologous bone marrow mesenchymal stem cells.
