中华损伤与修复杂志（电子版）
中華損傷與脩複雜誌（電子版）
중화손상여수복잡지（전자판）
Chinese Journal of Injury Repair and Wound Healing
2009年
2期
147-150
,共4页
转化生长因子-β%胰岛素样生长因子-I%软骨细胞%软骨缺损
轉化生長因子-β%胰島素樣生長因子-I%軟骨細胞%軟骨缺損
전화생장인자-β%이도소양생장인자-I%연골세포%연골결손
transforming growth factor-β%insulin-like growth factor-I%chondrocyte%cartilage defect
目的 观察转化生长因子-β(transforming growth factor-β,TGF-β)、胰岛素样生长因子-I(insulin-like growth factor-I,IGF-I)对关节软骨缺损修复的作用.方法 采用组织工程方法制备骨基质明胶(BMG)软骨细胞移植物.将40只4月龄的新西兰兔随机分为TGF-β组、IGF-I组、TGF-β联合IGF-I组、空白对照组(前三组为实验组,).各组制备关节软骨缺损模型,实验组兔膝关节腔注射对应等量人重组蛋白,对照组注射等量盐水.术后行组织学观察及免疫组化检测.结果 TGF-β联合IGF-I组软骨细胞生长较快,术后24周修复的软骨组织HE染色与正常关节软骨一致,软骨细胞呈柱状排列,免疫组化见Ⅱ型胶原染色较深;TGF-β组、IGF-I组术后24周部分软骨细胞呈柱状排列,免疫组化见Ⅱ型胶原染色较浅;空白对照组未修复.结论 联合应用TGF-β及IGF-I可较好促进关节软骨缺损修复,其作用优于两者单独应用.
目的 觀察轉化生長因子-β(transforming growth factor-β,TGF-β)、胰島素樣生長因子-I(insulin-like growth factor-I,IGF-I)對關節軟骨缺損脩複的作用.方法 採用組織工程方法製備骨基質明膠(BMG)軟骨細胞移植物.將40隻4月齡的新西蘭兔隨機分為TGF-β組、IGF-I組、TGF-β聯閤IGF-I組、空白對照組(前三組為實驗組,).各組製備關節軟骨缺損模型,實驗組兔膝關節腔註射對應等量人重組蛋白,對照組註射等量鹽水.術後行組織學觀察及免疫組化檢測.結果 TGF-β聯閤IGF-I組軟骨細胞生長較快,術後24週脩複的軟骨組織HE染色與正常關節軟骨一緻,軟骨細胞呈柱狀排列,免疫組化見Ⅱ型膠原染色較深;TGF-β組、IGF-I組術後24週部分軟骨細胞呈柱狀排列,免疫組化見Ⅱ型膠原染色較淺;空白對照組未脩複.結論 聯閤應用TGF-β及IGF-I可較好促進關節軟骨缺損脩複,其作用優于兩者單獨應用.
목적 관찰전화생장인자-β(transforming growth factor-β,TGF-β)、이도소양생장인자-I(insulin-like growth factor-I,IGF-I)대관절연골결손수복적작용.방법 채용조직공정방법제비골기질명효(BMG)연골세포이식물.장40지4월령적신서란토수궤분위TGF-β조、IGF-I조、TGF-β연합IGF-I조、공백대조조(전삼조위실험조,).각조제비관절연골결손모형,실험조토슬관절강주사대응등량인중조단백,대조조주사등량염수.술후행조직학관찰급면역조화검측.결과 TGF-β연합IGF-I조연골세포생장교쾌,술후24주수복적연골조직HE염색여정상관절연골일치,연골세포정주상배렬,면역조화견Ⅱ형효원염색교심;TGF-β조、IGF-I조술후24주부분연골세포정주상배렬,면역조화견Ⅱ형효원염색교천;공백대조조미수복.결론 연합응용TGF-β급IGF-I가교호촉진관절연골결손수복,기작용우우량자단독응용.
Objective To observe the effect of transforming growth factor-β and insulin-like growth factor-I on the repair of articular cartilage defects.Methods Bone matrix gelatin(BMG)engineered chondrocytes were implanted into the articular cartilage defects on the allogeneic rabbit knee joint.TGF-β and IGF-I were injected into the knee cavity in the experiment group,saline was injected in the control group.The histologic staining and immunohistochemistry were performed postoperatively.Results Chondrocytes arrangement,histologic staining, and expression of Type 2 collagen of the repaired cartilage tissues in the TGF-β combining with IGF-I group were found to be better than the other groups.The repaired cartilage tissues in TGF-β combining with IGF-I group were found to be similar to the normal articular cartilage 24 weeks after operation.The blank control group was not repaired.Conclusions TGF-β combining with IGF-I can promote the repair of articular cartilage defects.and the effect is better than TGF-β and IGF-I alone respectively.
