CAJ | 학술논문

建立了同时测定喜树叶片中喜树碱含量的高效液相色谱分析方法.采用sinochrom ODS C18分析柱(250 mm×4.6 mm BP,5 μm),以甲醇/水为流动相,检测波长为266 nm,梯度洗脱程序为:在前6 min流动相甲醇-水的体积比由40%线性增加至50%, 并保持恒定2 min, 在随后的32 min甲醇-水的体积比线性增至70%,在40 min时停止该程序;喜树碱在低、中、高3个量的平均加样回收率分别为99.66%,100.3%,99.97%; 10-羟基喜树碱在低、中、高3个量的平均加样回收率分别为99.87%,99.98%,100.4%.对不同季节的喜树嫩叶和成熟叶进行了喜树碱与10-羟基喜树碱含量的分析,结果表明喜树碱和10-羟基喜树碱的含量变化呈现明显的相关性.
건립료동시측정희수협편중희수감함량적고효액상색보분석방법.채용sinochrom ODS C18분석주(250 mm×4.6 mm BP,5 μm),이갑순/수위류동상,검측파장위266 nm,제도세탈정서위:재전6 min류동상갑순-수적체적비유40%선성증가지50%, 병보지항정2 min, 재수후적32 min갑순-수적체적비선성증지70%,재40 min시정지해정서;희수감재저、중、고3개량적평균가양회수솔분별위99.66%,100.3%,99.97%; 10-간기희수감재저、중、고3개량적평균가양회수솔분별위99.87%,99.98%,100.4%.대불동계절적희수눈협화성숙협진행료희수감여10-간기희수감함량적분석,결과표명희수감화10-간기희수감적함량변화정현명현적상관성.
An HPLC method for the simultaneous determination of CPT and HCPT in Camptotheca acuminata Decne. Leaves was established. Chromatography was performed with a sinochrom ODS column, monitored at 266 nm and the mobile phase was gradient H2O/CH3OH. The solvent gradient program was as follow: methanol-water increased linearly from 40% to 50% at the first 6 min and retained for 2 min with 50%, then increased linearly to 70% in the next 32 min. The average recoveries of CPT in three levels were 100.02%, 98.94% and 99.25%, The average recoveries of HCPT were 99.13%, 99.66% and 98.97%. Using this assay, CPT and HCPT in leaves of Camptotheca acuminata Decne. In different seasons were determined. The results showed that the accumulation of CPT and HCPT presented evident relativity in the different growth stages.