CAJ | 학술논문

目的:探讨中药提取物沙苑子黄酮(flavonoids extracts from semen Astragali complanati,FAC)对小鼠肝癌H22细胞生长的影响及其机制.方法:建立H22荷瘤小鼠模型,观察FAC对H22肝癌移植瘤生长的抑制作用及其对荷瘤小鼠免疫器官、巨噬细胞吞噬功能、淋巴细胞转化功能及生存期的影响.结果:高、中、低剂量组FAC对荷瘤小鼠肝癌细胞生长具有显著抑制作用,与正常对照模型组比较差异具有统计学意义(P<0.05);其中环磷酰胺(cyclophosphamide,CTX)对照组的抑瘤率与FAC高剂量组相近(P>0.05). FAC可以明显延长荷瘤小鼠的存活期,高、中、低剂量组的生命延长率分别为64.9%、56.7%和28.1%,与CTX对照组比较差异具有统计学意义(P<0.01).另外,高、中、低剂量组FAC明显提高荷瘤小鼠的胸腺指数和脾指数,与正常对照模型组比较差异具有统计学意义(P<0.05);且高、中剂量组能显著提高巨噬细胞吞噬功能和淋巴细胞转化能力,与正常对照模型组比较差异具有统计学意义(P<0.01);而CTX的作用方向相反,与正常对照模型组比较差异也具有统计学意义(P<0.01).结论:FAC可抑制肝癌H22细胞生长,延长荷瘤小鼠存活期,并能提高荷瘤小鼠的非特异性免疫功能.FAC可能通过调节机体免疫功能来发挥抗肿瘤作用.
목적:탐토중약제취물사원자황동(flavonoids extracts from semen Astragali complanati,FAC)대소서간암H22세포생장적영향급기궤제.방법:건립H22하류소서모형,관찰FAC대H22간암이식류생장적억제작용급기대하류소서면역기관、거서세포탄서공능、림파세포전화공능급생존기적영향.결과:고、중、저제량조FAC대하류소서간암세포생장구유현저억제작용,여정상대조모형조비교차이구유통계학의의(P<0.05);기중배린선알(cyclophosphamide,CTX)대조조적억류솔여FAC고제량조상근(P>0.05). FAC가이명현연장하류소서적존활기,고、중、저제량조적생명연장솔분별위64.9%、56.7%화28.1%,여CTX대조조비교차이구유통계학의의(P<0.01).령외,고、중、저제량조FAC명현제고하류소서적흉선지수화비지수,여정상대조모형조비교차이구유통계학의의(P<0.05);차고、중제량조능현저제고거서세포탄서공능화림파세포전화능력,여정상대조모형조비교차이구유통계학의의(P<0.01);이CTX적작용방향상반,여정상대조모형조비교차이야구유통계학의의(P<0.01).결론:FAC가억제간암H22세포생장,연장하류소서존활기,병능제고하류소서적비특이성면역공능.FAC가능통과조절궤체면역공능래발휘항종류작용.
Objective:To study the effect of flavonoids extracts from semen Astragali complanati (FAC) on the growth of hepatocellular H22 cells and elucidate its action mechanism. Methods:The mouse model bearing H22 tumor cells was established. The effects of FAC on the growth of xenografted H22 tumor, the immune organ, survival time, phagocytic function of macrophages, and lymphocyte transformation in tumor-bearing mice were observed. Results:The growth of H22 transplanted tumor was significantly inhibited by FAC at high, middle and low doses,compared with normal control group (P<0.05). The tumor-inhibiting ratio in cyclophosphamide (CTX) group was similar with that in FAC high-dose group (P>0.05). FAC markedly elongated the survival time of tumor-bearing mice. The high, middle and low doses of FAC elongated the survival time of tumor-bearing mice by 64.9%, 56.7% and 28.1%, which were significantly different with control group (P<0.01). The high, middle and low doses of FAC greatly increased the thymus index and spleen index of tumor-bearing mice (P<0.05, vs control) and elevated the phagocytic function of macrophages and lymphocyte transformation capability (P<0.01, vs control). The effect of CTX on immune function of tumor-bearing mice was opposite with FAC. The difference between CTX group and control group was significant (P<0.01). Conclusion: FAC inhibits the growth of H22 hepatoma, elongates the survival time, and elevates the non-specific immune function of tumor-bearing mice, indicating that FAC maybe exert its anti-tumor effect via regulating immune function of tumor-bearing mice.