CAJ | 학술논문

蚕豆锈病由蚕豆单孢锈菌(Uromyces fabae de Bary)引起,是一种常见专性寄生的真菌病害,其侵染和传播方式与大豆锈病(Phakopsora pachyrhiri Syd.)相同,主要以夏孢子传播.因此常作为大豆锈病研究的模式靶标.又因其无法在人工培养基上生长繁殖,而基于植株的筛选方式难以适应先导化舍物筛选.该文针对蚕豆锈病离体叶片人工接种技术进行了探索,对影响蚕豆锈病侵染的主要因素进行了研究,建立了基于离体叶片法筛选的先导化合物筛选方法.采用离体叶片孢子悬浮液喷雾法对1 522份真菌固体发酵提取物和放线菌液体摇瓶发酵提取物进行了初筛,筛选出具有杀菌活性的提取物90份.对经过HPLC纯化后的540个组分进行复筛,获得具较高杀菌活性的化合物3个. 筛选结果表明该筛选方法能稳定、高效地进行先导化合物筛选.
잠두수병유잠두단포수균(Uromyces fabae de Bary)인기,시일충상견전성기생적진균병해,기침염화전파방식여대두수병(Phakopsora pachyrhiri Syd.)상동,주요이하포자전파.인차상작위대두수병연구적모식파표.우인기무법재인공배양기상생장번식,이기우식주적사선방식난이괄응선도화사물사선.해문침대잠두수병리체협편인공접충기술진행료탐색,대영향잠두수병침염적주요인소진행료연구,건립료기우리체협편법사선적선도화합물사선방법.채용리체협편포자현부액분무법대1 522빈진균고체발효제취물화방선균액체요병발효제취물진행료초사,사선출구유살균활성적제취물90빈.대경과HPLC순화후적540개조분진행복사,획득구교고살균활성적화합물3개. 사선결과표명해사선방법능은정、고효지진행선도화합물사선.
Broadbean rust is a kind of obhgateparasite.The methods of infecting and spreading are similar with soybean rust (Phakopsora pochyrhizi syd).So it is always used as the model target of soybean rust.The culture of this pathogen is not progressing in media,so the screening of lead compounds confined in plants.The artificial inoculation of broadbean rust had been studied on leaf-dish.The factors of effect on infection of broadbean rust had been studied.The program of screening method was found based on method of the leaf-dish. 1 522 extracts from microorganism had been tested by the screening method,90 extracts showed fungicidal activity.540 samples formulated from profiling by HPLC had been tested,and their lead compounds had been found.The screening results showed that the screening method Was stable and efficient.