CAJ | 학술논문

介绍了一种基于化学发光磁酶免疫分析技术测定人绒毛膜促性腺激素(HCG)的检测系统.该检测系统通过良好的屏蔽和抗干扰设计,以高速高频的FPGA器件完成光子计数,实现对HCG的定量检测.对HCG抗原溶液的检测结果表明,检测系统在HCG抗原浓度为0.06～600 ng/mL范围内线性关系良好,相关系数R=0.951.对检测系统进行重复性测试,变异系数为0.61%,重复性较好.该系统具有小型便携、灵敏度高、重复性好等特点,在疾病标志物现场检测领域具有较为广阔的应用前景.
개소료일충기우화학발광자매면역분석기술측정인융모막촉성선격소(HCG)적검측계통.해검측계통통과량호적병폐화항간우설계,이고속고빈적FPGA기건완성광자계수,실현대HCG적정량검측.대HCG항원용액적검측결과표명,검측계통재HCG항원농도위0.06～600 ng/mL범위내선성관계량호,상관계수R=0.951.대검측계통진행중복성측시,변이계수위0.61%,중복성교호.해계통구유소형편휴、령민도고、중복성호등특점,재질병표지물현장검측영역구유교위엄활적응용전경.
An HCG detecting system based on chemiluminescent magnetic enzyme-linked immunoassay is described. The system could detect weak chemiluminescence sensitively using photon-counting method with a special photo-electronic detection unit. In order to improve the detecting precision, FPGA is used to count the photon pulse signal. HCG sample with concentration of 0.06～600 ng/mL was measured and the correlation coefficient between HCG concentrations is 0.951. The variation coefficient of the system is 0.61%. With high sensitivity and high precision, the system will have great potential in trace disease marker measurement.