CAJ | 학술논문

目的对病死仔猪肠道内分离的大肠杆菌进行分离培养,通过常规生化反应及毒力基因和分型基因的分子生物学检测对分离株进行初步鉴定.方法应用多重PCR 检测方法进行毒力基因的检测,经细菌16S rDNA全基因扩增测序,Flic基因扩增测序,Blast分析,对分离的大肠杆菌进行初步分型鉴定.结果通过对分离菌株基因组DNA的多重PCR分析,发现该菌株含大肠杆菌耐热肠毒素和不耐热肠毒素致病基因.通过细菌16S rDNA全基因扩增测序,Flic基因扩增测序,Blast分析显示该分离株为H10.其中16S rDNA全基因与H10菌株同源性在99%,Flic基因与H10菌株同源性在98%.结论所分离的猪肠道致病性大肠杆菌为H10,其含耐热肠毒素和不耐热肠毒素致病基因.
목적 대병사자저장도내분리적대장간균진행분리배양,통과상규생화반응급독력기인화분형기인적분자생물학검측대분리주진행초보감정.방법 응용다중PCR 검측방법진행독력기인적검측,경세균16S rDNA전기인확증측서,Flic기인확증측서,Blast분석,대분리적대장간균진행초보분형감정.결과 통과대분리균주기인조DNA적다중PCR분석,발현해균주함대장간균내열장독소화불내열장독소치병기인.통과세균16S rDNA전기인확증측서,Flic기인확증측서,Blast분석현시해분리주위H10.기중16S rDNA전기인여H10균주동원성재99%,Flic기인여H10균주동원성재98%.결론 소분리적저장도치병성대장간균위H10,기함내열장독소화불내열장독소치병기인.
The Escherichia coli (E.coli) strain from intestinal tract of died newborn swine was isolated and cultured.Preliminary identification of the isolated strain was conducted by conventional biochemical tests,and the molecular biology detections of toxicity gene and typing gene were completed by multi-PCR.Stable toxin and heat-labile enterotoxin genes of E.coli were detected from the isolated strain.By amplifying and sequencing bacterial 16s rDNA and fliC gene,the isolated strain was identified as H10 by Blast analysis.The homology of strain H10 was 99% with bacterial 16s rDNA gene and 98% with fliC gene.