CAJ | 학술논문

目的:建立分析肝癌患者血浆/血清中低丰度差异表达蛋白质的双向凝胶电泳(2-DE)方法.方法:以10例肝癌患者血浆、8例肝癌患者血清及8例正常志愿者血浆和血清为研究对象,选用安捷伦免疫亲和色谱Agilent MARS Spin Cartridge去除血浆中6种主要高丰度蛋白,2-DE分离血浆/血清低丰度蛋白质,差异蛋白质斑点经酶解后,MALDI-TOF/MS检测的肽指纹谱(Peptide mass fingerprinting,PMF)在NCBInr数据库中进行搜索.结果:10例肝癌患者血浆样品中共鉴定出有20种差异蛋白,其中18种表达上调,2种表达.8例肝癌患者血清样品中共鉴定出23种差异蛋白,其中17种蛋白表达上调,6种蛋白表达下调.在肝癌患者血清和血浆组中共同识别到2种差异蛋向质.结论:血浆组中识别到的指环蛋白34(RING finger protein34),威尔姆肿瘤1-关联蛋白(Wilms'tumor 1-associating),CD147抗原,卵泡前体FS(Follistatin precursor,FS)蛋白和血清组中识别到的蛋白激酶NYD-SP9(Protein kinase NYD-SP9),肿瘤坏死因子受体超家族成员19前体(Tumor necrosis factor receptor superfamily member 19precursor),组蛋白键合蛋白RBBP4(Histone-binding protein RBBP4),FADD蛋白,26S蛋白酶调控亚基P28(26S proteasome regulatory subunit p28),CD82抗原,自体吞噬蛋白5(Autophagy protein 5)等蛋白有望成为(Hepato celluar careinoma,HCC)的特异蛋白.
목적:건립분석간암환자혈장/혈청중저봉도차이표체단백질적쌍향응효전영(2-DE)방법.방법:이10례간암환자혈장、8례간암환자혈청급8례정상지원자혈장화혈청위연구대상,선용안첩륜면역친화색보Agilent MARS Spin Cartridge거제혈장중6충주요고봉도단백,2-DE분리혈장/혈청저봉도단백질,차이단백질반점경매해후,MALDI-TOF/MS검측적태지문보(Peptide mass fingerprinting,PMF)재NCBInr수거고중진행수색.결과:10례간암환자혈장양품중공감정출유20충차이단백,기중18충표체상조,2충표체.8례간암환자혈청양품중공감정출23충차이단백,기중17충단백표체상조,6충단백표체하조.재간암환자혈청화혈장조중공동식별도2충차이단향질.결론:혈장조중식별도적지배단백34(RING finger protein34),위이모종류1-관련단백(Wilms'tumor 1-associating),CD147항원,란포전체FS(Follistatin precursor,FS)단백화혈청조중식별도적단백격매NYD-SP9(Protein kinase NYD-SP9),종류배사인자수체초가족성원19전체(Tumor necrosis factor receptor superfamily member 19precursor),조단백건합단백RBBP4(Histone-binding protein RBBP4),FADD단백,26S단백매조공아기P28(26S proteasome regulatory subunit p28),CD82항원,자체탄서단백5(Autophagy protein 5)등단백유망성위(Hepato celluar careinoma,HCC)적특이단백.