中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2012年
5期
278-282
,共5页
脓毒症%肝素%内皮细胞通透性%细胞骨架%内皮细胞纤维状肌动蛋白
膿毒癥%肝素%內皮細胞通透性%細胞骨架%內皮細胞纖維狀肌動蛋白
농독증%간소%내피세포통투성%세포골가%내피세포섬유상기동단백
Sepsis%Heparin%Endothelial cell permeability%Cytoskeleton%F-actin
目的 研究肝素对脂多糖(LPS)诱导入脐静脉内皮细胞(HUVECs)单层通透性和细胞骨架形态的影响.方法 HUVECs细胞株传代培养后随机分为空白对照组、LPS组、肝素组、LPS+肝素组,n=8.用四甲基偶氮唑盐(MTT)比色法测定内皮细胞活性；用Transwell小室法测定单层内皮细胞通透性；用免疫荧光染色法测定内皮细胞纤维状肌动蛋白(F-actin)形态.结果 与空白对照组比较,LPS 10 mg/L及100 mg/L对细胞活性起到明显的抑制作用(0.695±0.015、0.476±0.030比0.860±0.053,P＜0.05和P＜0.01),肝素100 kU/L对内皮细胞活性产生抑制作用(0.675±0.030比0.840±0.023,P＜0.05).LPS10mg/L可增加单层内皮细胞通透性,于4h时达峰值,与空白对照组比较差异有统计学意义(5.882±0.101比4.489±0.015,P＜0.05),并能增加细胞骨架应力纤维的形成；而LPS+肝素刺激2～12h时单层内皮细胞的通透性较LPS组明显降低(2 h:4.382±0.053比5.084±0.129,4 h:4.528±0.044比5.882±0.101,6 h:4.381±0.089比5.479±0.125,12 h:4.447±0.054比4.719±0.080,均P＜0.05),并能减少内皮细胞骨架的重排及应力纤维的形成.结论 肝素可以减轻LPS诱导单层内皮细胞通透性增高及细胞骨架重排,对内皮细胞屏障功能起到保护作用.
目的 研究肝素對脂多糖(LPS)誘導入臍靜脈內皮細胞(HUVECs)單層通透性和細胞骨架形態的影響.方法 HUVECs細胞株傳代培養後隨機分為空白對照組、LPS組、肝素組、LPS+肝素組,n=8.用四甲基偶氮唑鹽(MTT)比色法測定內皮細胞活性；用Transwell小室法測定單層內皮細胞通透性；用免疫熒光染色法測定內皮細胞纖維狀肌動蛋白(F-actin)形態.結果 與空白對照組比較,LPS 10 mg/L及100 mg/L對細胞活性起到明顯的抑製作用(0.695±0.015、0.476±0.030比0.860±0.053,P＜0.05和P＜0.01),肝素100 kU/L對內皮細胞活性產生抑製作用(0.675±0.030比0.840±0.023,P＜0.05).LPS10mg/L可增加單層內皮細胞通透性,于4h時達峰值,與空白對照組比較差異有統計學意義(5.882±0.101比4.489±0.015,P＜0.05),併能增加細胞骨架應力纖維的形成；而LPS+肝素刺激2～12h時單層內皮細胞的通透性較LPS組明顯降低(2 h:4.382±0.053比5.084±0.129,4 h:4.528±0.044比5.882±0.101,6 h:4.381±0.089比5.479±0.125,12 h:4.447±0.054比4.719±0.080,均P＜0.05),併能減少內皮細胞骨架的重排及應力纖維的形成.結論 肝素可以減輕LPS誘導單層內皮細胞通透性增高及細胞骨架重排,對內皮細胞屏障功能起到保護作用.
목적 연구간소대지다당(LPS)유도입제정맥내피세포(HUVECs)단층통투성화세포골가형태적영향.방법 HUVECs세포주전대배양후수궤분위공백대조조、LPS조、간소조、LPS+간소조,n=8.용사갑기우담서염(MTT)비색법측정내피세포활성；용Transwell소실법측정단층내피세포통투성；용면역형광염색법측정내피세포섬유상기동단백(F-actin)형태.결과 여공백대조조비교,LPS 10 mg/L급100 mg/L대세포활성기도명현적억제작용(0.695±0.015、0.476±0.030비0.860±0.053,P＜0.05화P＜0.01),간소100 kU/L대내피세포활성산생억제작용(0.675±0.030비0.840±0.023,P＜0.05).LPS10mg/L가증가단층내피세포통투성,우4h시체봉치,여공백대조조비교차이유통계학의의(5.882±0.101비4.489±0.015,P＜0.05),병능증가세포골가응력섬유적형성；이LPS+간소자격2～12h시단층내피세포적통투성교LPS조명현강저(2 h:4.382±0.053비5.084±0.129,4 h:4.528±0.044비5.882±0.101,6 h:4.381±0.089비5.479±0.125,12 h:4.447±0.054비4.719±0.080,균P＜0.05),병능감소내피세포골가적중배급응력섬유적형성.결론 간소가이감경LPS유도단층내피세포통투성증고급세포골가중배,대내피세포병장공능기도보호작용.
Objective To evaluate the effects of heparin on the changes in permeability and cytoskeleton of cultured human umbilical vascular endothelial cells (HUVECs) induced by lipopolysaccharide (LPS).Methods HUVECs were cultured in vitro and randomly assigned to blank control group,LPS group,heparin group,and LPS + heparin group,n =8.Cell viahility was determined by methyl thiazolyl tetrazolium (MTT) method.Endothelial permeability was measured with the Transwell chamber models.F-actin of cytoskeletons was assayed with fluorescein isothiocyanate (FITC)-phalloidine.Results Compared with the blank control group,10 mg/L and 100 mg/L of LPS significantly inhibited cell viability (0.695 ± 0.015,0.476 ± 0.030 vs.0.860 ± 0.053,P＜0.05 and P＜0.01 ).Heparin 100 kU/L also inhibited cell viability (0.675 ± 0.030 vs.0.840 ± 0.023,P ＜0.05 ).Increase in permeability of endothelium was induced by 10 mg/L of LPS,and it peaked at 4 hours,there was significant difference compared with blank control group (5.882 ± 0.101 vs.4.489 ± 0.015,P＜0.05 ).At the same time,LPS led to the reorganization of F-actin cytoskeleton and the fiormation of stress fibers.LPS + heparin decreased the increase in permeability of endothelium at 2-12 hours (2 hours:4.382 ± 0.053 vs.5.084 ± 0.129,4 hours:4.528 ± 0.044 vs.5.882 ± 0.101,6 hours:4.381 ± 0.089 vs.5.479 ± 0.125,12 hours:4.447 ± 0.054 vs.4.719 ± 0.080,all P＜0.05 ),and attenuated the reorganization of F-actin and the formation of stress fibers.Conclusion Heparin attenuates LPS-induced increase in permeability of HUVECs and alterations in F-actin organization,thus protects endothelial barrier.
