CAJ | 학술논문

目的探讨一种新型的免缝合光化学组织粘合术(photochemical tissue bonding,PTB)修复角膜穿透伤的方法,并评价其疗效. 方法 60只新西兰大白兔建角膜穿透伤模型,伤后左眼用缝合法修复损伤,右眼以PTB技术固定羊膜封闭创面,伤后不同时相点观察眼前房液渗漏清况及前房形态；评价眼内压情况；取角膜组织行组织学检测,观察角膜的新生血管和瘢痕形成,比较两种方法的修复效果. 结果两组均未见眼房水渗漏,且眼前房深浅均正常无差别；PTB组见羊膜与角膜贴合紧密.PTB治疗后0,1,7d最大眼内压分别为(531.2±49.5) mm Hg、(542.6±74.8) mm Hg、(603.9±69.1) mm Hg,明显优于缝合组[(41.3±12.7) mmHg、(142.6±25.4) mm Hg、(333.3 ±66.7) mm Hg](P＜0.01).PTB组角膜新生血管和肉芽形成在伤后14,28及90 d时明显少于缝合组. 结论 PTB结合羊膜是一种即时、有效封闭角膜穿透伤的方法,且修复效果明显优于缝合法.
목적 탐토일충신형적면봉합광화학조직점합술(photochemical tissue bonding,PTB)수복각막천투상적방법,병평개기료효. 방법 60지신서란대백토건각막천투상모형,상후좌안용봉합법수복손상,우안이PTB기술고정양막봉폐창면,상후불동시상점관찰안전방액삼루청황급전방형태；평개안내압정황；취각막조직행조직학검측,관찰각막적신생혈관화반흔형성,비교량충방법적수복효과. 결과 량조균미견안방수삼루,차안전방심천균정상무차별；PTB조견양막여각막첩합긴밀.PTB치료후0,1,7d최대안내압분별위(531.2±49.5) mm Hg、(542.6±74.8) mm Hg、(603.9±69.1) mm Hg,명현우우봉합조[(41.3±12.7) mmHg、(142.6±25.4) mm Hg、(333.3 ±66.7) mm Hg](P＜0.01).PTB조각막신생혈관화육아형성재상후14,28급90 d시명현소우봉합조. 결론 PTB결합양막시일충즉시、유효봉폐각막천투상적방법,차수복효과명현우우봉합법.
Objective To develop a new sutureless technique (photochemical tissue bonding,PTB ) for repair of corneal perforation. Methods A total of 60 rabbits were used for creating corneal perforation models.The corneal perforation on the left eye was repaired by sutures and the injury on the right eye was fixed with the use of amniotic membrane with PTB.The outcomes of the two mentioned repair methods were compared by observing the leakage of aqueous and the morphology of the anterior chamber at different instants,measuring the intraocular pressure (IOP) and observing the formation of neo-vessels and scars of cornea in the use of histological analysis. Results There was no leakage of aqueous and no difference for morphology evaluation in both treatments.PTB could adhere AM on the cornea to restore the corneal perforation.The peak IOP in the PTB treatment group at days 0,7 and 14 postoperative [ (531.2 ±49.5) mm Hg,(542.6 ±74.8) mm Hg and (603.9 ±69.1) mm Hg,respectively] was significantly higher than that in the suture group at the same instants [ (41.3 ±12.7) mm Hg,(142.6 ±25.4) mm Hg and (333.3 ± 66.7) mm Hg,respectively] (P ＜0.O1 ).Compared with suture repair,the treatment with PTB resulted in a better outcome of wound healing with less neo-vessels and less scars of cornea. Conclusion PTB treatment for repair of corneal perforation is superior to suture repair.