CAJ | 학술논문

目的研究组织工程脊髓移植治疗大鼠脊髓半切块状损伤的疗效.方法以聚乳酸-羟基乙酸(PLGA)为细胞支架,多聚赖氨酸为细胞外基质,神经十细胞(NSCs)为种子细胞,体外构建组织工程脊髓.制作大鼠T10脊髓右半切块状损伤模型,随机分成3组:实验组在损伤区移植组织工程脊髓,对照组A移植NSCs,对照组B移植PLGA.移植治疗12周,每周均行BBB评分定量评价肢体运动功能.伤后第12周辣根过氧化物酶(HRP)神经逆行示踪评价脊髓传导束的恢复程度,并取损伤处脊髓组织行免疫组织化学染色,观察移植区的形态结构修复.结果伤后12周实验组的BBB运动功能评分较对照组明显提高,差异有统计学意义(P＜0.05).HRP神经逆行示踪显示:实验组鼠右侧大脑组织中可见大量的HRP标记阳性神经元,而两对照组仅见有少量HRP阳性神经元;免疫组织化学染色显示:实验组移植区NF阳性神经元和GAP-43阳性神经轴索数量较多,修复了缺损,而对照组极少,仍留下不同程度的缺损.结论组织工程脊髓移植治疗促进了半切块状损伤脊髓的形态结构修复和功能恢复,疗效明显优于单纯的NSCs移植和PLGA移植.
목적 연구조직공정척수이식치료대서척수반절괴상손상적료효.방법 이취유산-간기을산(PLGA)위세포지가,다취뢰안산위세포외기질,신경십세포(NSCs)위충자세포,체외구건조직공정척수.제작대서T10척수우반절괴상손상모형,수궤분성3조:실험조재손상구이식조직공정척수,대조조A이식NSCs,대조조B이식PLGA.이식치료12주,매주균행BBB평분정량평개지체운동공능.상후제12주랄근과양화물매(HRP)신경역행시종평개척수전도속적회복정도,병취손상처척수조직행면역조직화학염색,관찰이식구적형태결구수복.결과 상후12주실험조적BBB운동공능평분교대조조명현제고,차이유통계학의의(P＜0.05).HRP신경역행시종현시:실험조서우측대뇌조직중가견대량적HRP표기양성신경원,이량대조조부견유소량HRP양성신경원;면역조직화학염색현시:실험조이식구NF양성신경원화GAP-43양성신경축색수량교다,수복료결손,이대조조겁소,잉류하불동정도적결손.결론 조직공정척수이식치료촉진료반절괴상손상척수적형태결구수복화공능회복,료효명현우우단순적NSCs이식화PLGA이식.
Objective To investigate the effects of transplantation of tissue engineering spinal cord on spinal cord half-cut lump injury in rats.Methods Tissue engineering spinal cord was constructed in vitro by using PLGA(Poly lactic-co-glycolic acid)scaffold and neural stem cells.Animal model of a halfcut lump spinal cord was made in T10.The animals were divided into 3 groups randomly:experiment group transplanted with tissue engineering spinal cord,control group A injected with neural stem cells,and control group B positioned with scaffold.The effects of transplantation with tissue engineering spinal cord were evaluated by the BBB scale、HRP tracing technique and immunohistochemistry respectively.Results The average BBB SCales in the experiment group were higher than that in the control groups,and the differences had significance.Significant amount of HRP positive neurons were observed in the experiment group compared to the two control groups.Furthermore,immunohistoehemistry showed that more NF positive and GAP-43 positive nerve fibers exhibited in the vicinity of the injury region in the experiment group,less positive neurons and more fibers were distrihuted in the two control groups.Conclusion Transplantation of tissue engineering spinal cord can promote the structure repairing and functional recovery of half-cut lump spinal cord injury,and curative effect is better than transplantaion of neural stem cells or scaffold.