CAJ | 학술논문

目的分析外周T细胞淋巴瘤穿刺标本中T细胞受体(TCR)β链克隆性基因重排及互补决定区3(CDR3)谱型.方法应用RT-PCR扩增TCR V β 24个亚家族的CDR3基因,并经基因扫描确定T细胞克隆性,对提示单克隆或寡克隆增生亚家族的PCR产物测序分析其CDR3序列.结果 4例淋巴瘤患者TCR表达受到明显抑制,仅表达1～4个Vβ亚家族.所有患者均存在1个或多个Vβ亚家族的克隆增生T细胞,增生形式包括单克隆、双克隆及寡克隆增生趋势.CDR3区序列分析证实增生的T细胞克隆具有不同的氨基酸序列.结论外周T细胞淋巴瘤患者存在T细胞克隆性增生,其TCR V β呈限制性取用,不同克隆T细胞具有不同的CDR3谱型.
목적 분석외주T세포림파류천자표본중T세포수체(TCR)β련극륭성기인중배급호보결정구3(CDR3)보형.방법 응용RT-PCR확증TCR V β 24개아가족적CDR3기인,병경기인소묘학정T세포극륭성,대제시단극륭혹과극륭증생아가족적PCR산물측서분석기CDR3서렬.결과 4례림파류환자TCR표체수도명현억제,부표체1～4개Vβ아가족.소유환자균존재1개혹다개Vβ아가족적극륭증생T세포,증생형식포괄단극륭、쌍극륭급과극륭증생추세.CDR3구서렬분석증실증생적T세포극륭구유불동적안기산서렬.결론 외주T세포림파류환자존재T세포극륭성증생,기TCR V β정한제성취용,불동극륭T세포구유불동적CDR3보형.
Objective To analyze the elonal gene rearrangement and complementarity determining region 3 (CDR3) Repertoire of TCR β-chain in fine needle aspiration biopsy (FNAB) specimens of peripheral T-cell lymphoma. Methods The TCR CDR3 region genes of 24 TCR Vβ subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 size lengths of TCR β-chain were analyzed with genesean technology for 4 healthy individuals and 4 patients with peripheral T-cell lymphoma. The clonality of T cells presumed by spectratyping was further confirmed by CDR3 sequencing. Results TCR β-chain presented specific repertoire skewing in 4 cases with peripheral T-cell lymphoma, and only 1-4 TCR Vβ subfamily T cells were identified, respectively. Clonal expanded T cells, including mono, bioclonal and oligoclonal trend patterns, in one or more Vβ subfamilies were found in all cases. The mono expanded T cells have different CDR3 amino acid sequences. Conclusion Characteristic T cells cloning proliferation and selected usage of TCR Vβ subfamily T cells were found in 4 cases with peripheral T-cell lymphoma. The sequences of CDR3 in different TCR clone proliferation are different.