CAJ | 학술논문

目的探讨淀粉样前体蛋白(APP)、裂殖1同源物(Fis1)、杀伤细胞凝集素样受体亚家族F成员1(KLRF1)和细胞程序性死亡7(PDCD7)mRNA在急性髓系白血病(AML)细胞中的表达及其预后意义.方法抽取34例AML及10例非恶性血液病患者骨髓.提取总RNA并逆转录成cDNA,SYBR Green I实时定量PCR法检测各基因mRNA表达水平,采用2-~(△Cr)公式计算基因mRNA相对表达量.结果 AML患者组PDCD7mRNA表达水平均显著高于对照组(Z=-2.213,P=0.027).除M1(1例)、M2a(2例)外的AML各亚型基因表达同对照组比较,APPmRNA在伴有t(8,21)染色体异常的M2表达显著高于对照组(Z=-2.197,P=0.028),而在M4b和M5b中的表达则显著低于对照组(Z=-2.368,P=0.018).APP mRNA在AML亚型间表达有差异,M2显著高于M4和M5(Z=-2.430,P=0.015;Z=-3.175,P=0.001).结论 AML患者高表达PDCD7基因,单核细胞白血病患者低表达APP基因.
목적 탐토정분양전체단백(APP)、렬식1동원물(Fis1)、살상세포응집소양수체아가족F성원1(KLRF1)화세포정서성사망7(PDCD7)mRNA재급성수계백혈병(AML)세포중적표체급기예후의의.방법 추취34례AML급10례비악성혈액병환자골수.제취총RNA병역전록성cDNA,SYBR Green I실시정량PCR법검측각기인mRNA표체수 평,채용2-~(△Cr)공식계산기인mRNA상대표체량.결과 AML환자조PDCD7mRNA표체수평균현저고우대조조(Z=-2.213,P=0.027).제M1(1례)、M2a(2례)외적AML각아형기인표체동대조조비교,APPmRNA재반유t(8,21)염색체이상적M2표체현저고우대조조(Z=-2.197,P=0.028),이재M4b화M5b중적표체칙현저저우대조조(Z=-2.368,P=0.018).APP mRNA재AML아형간표체유차이,M2현저고우M4화M5(Z=-2.430,P=0.015;Z=-3.175,P=0.001).결론 AML환자고표체PDCD7기인,단핵세포백혈병환자저표체APP기인.
Objective To investigate the expression of amyloid precursor protein (APP), Fis1, PDCD7 and KLRF1 mRNA in acute myeloid leukemia (AML) and their prognostic significances. Methods Thirty-four AML patients and 10 patients with nonmalignant hematologic diseases (control group) were recruited in this study. Bone marrows were obtained from these patients to isolate the mononuclear cells, from which total RNA was extracted and reverse transcribed into cDNA. SYBR Green Ⅰ dye-based real-time quantitative PCR method was used to compare the expression of APP, Fis1, PDCD7 and KLRF1 mRNA between two groups. Results The expression level of PDCD7 mRNA in AML patients was significantly higher than that in the control group (Z=-2.213,P=0.027), but APP, Fisl and KLRF1 mRNA expression levels were similar between the two groups (P<0.05). The expression level of APP mRNA in M2 patients with t (8, 21) chromosomal abnormality was significantly higher than that in the control group (Z=-2.197, P=0.028), while APP mRNA expressions in M4b and M5b patients were both lower than those in the control group (Z=-2.368, P-0.018). The expression of APP mRNA differed significantly between the subtypes of AML, higher in M2 than in M4b and M5b (Z=-2.430, P=0.015; Z=-3.175, P=0.001, respectively). Conclusions The expression of PDCD7 mRNA increased significantly in AML patients, and APP mRNA expression is decreased in monocytic leukemia.