重庆医科大学学报
重慶醫科大學學報
중경의과대학학보
UNIVERSITATIS SCIENTIAE MEDICINAE CHONGQING
2009年
12期
1679-1682
,共4页
丹参素%肝星状细胞%血小板衍生生长因子-BB%细胞外信号转导激酶1/2
丹參素%肝星狀細胞%血小闆衍生生長因子-BB%細胞外信號轉導激酶1/2
단삼소%간성상세포%혈소판연생생장인자-BB%세포외신호전도격매1/2
Danshensu%Hepatic stellate cell ( HSC )%Pdatelet derivde gnowth factor-BB (PDGF-BB)%Extracellular signal-regulated kinase 1/2(ERK1/2)
目的:观察丹参素对血小板衍生生长因子-BB(Platelet derived growth factor-BB,PDGF-BB)刺激大鼠肝星状细胞(Hepatic stellate cell,HSC)增殖、Ⅰ型胶原合成、磷酸化细胞外信号调节激酶1,2(Phosphoryhted extracellular signal-regulated kinase 1/2,P-ERK1/2)表达的影响,探讨丹参素抗肝纤维化的分子机制.方法:四甲基偶氮唑盐法检测大鼠HSC增殖活性;免疫细胞化学染色法测定大鼠HSC Ⅰ型胶原合成的表达;Western blot测定大鼠肝星状细胞P-ERK1/2的表达.结果:丹参素有抑制PDGF-BB刺激大鼠HSC增殖,Ⅰ型胶原表达,p-ERK1/2表达的作用.结论:丹参素可抑制PDGF-BB刺激的大鼠HSC增殖,Ⅰ型胶原合成,其机制可能与抑制ERK1/2信号转导通路有关.
目的:觀察丹參素對血小闆衍生生長因子-BB(Platelet derived growth factor-BB,PDGF-BB)刺激大鼠肝星狀細胞(Hepatic stellate cell,HSC)增殖、Ⅰ型膠原閤成、燐痠化細胞外信號調節激酶1,2(Phosphoryhted extracellular signal-regulated kinase 1/2,P-ERK1/2)錶達的影響,探討丹參素抗肝纖維化的分子機製.方法:四甲基偶氮唑鹽法檢測大鼠HSC增殖活性;免疫細胞化學染色法測定大鼠HSC Ⅰ型膠原閤成的錶達;Western blot測定大鼠肝星狀細胞P-ERK1/2的錶達.結果:丹參素有抑製PDGF-BB刺激大鼠HSC增殖,Ⅰ型膠原錶達,p-ERK1/2錶達的作用.結論:丹參素可抑製PDGF-BB刺激的大鼠HSC增殖,Ⅰ型膠原閤成,其機製可能與抑製ERK1/2信號轉導通路有關.
목적:관찰단삼소대혈소판연생생장인자-BB(Platelet derived growth factor-BB,PDGF-BB)자격대서간성상세포(Hepatic stellate cell,HSC)증식、Ⅰ형효원합성、린산화세포외신호조절격매1,2(Phosphoryhted extracellular signal-regulated kinase 1/2,P-ERK1/2)표체적영향,탐토단삼소항간섬유화적분자궤제.방법:사갑기우담서염법검측대서HSC증식활성;면역세포화학염색법측정대서HSC Ⅰ형효원합성적표체;Western blot측정대서간성상세포P-ERK1/2적표체.결과:단삼소유억제PDGF-BB자격대서HSC증식,Ⅰ형효원표체,p-ERK1/2표체적작용.결론:단삼소가억제PDGF-BB자격적대서HSC증식,Ⅰ형효원합성,기궤제가능여억제ERK1/2신호전도통로유관.
Objective:To investigate the inhibitory effect of Danshengsu on proliferation, collagen type Ⅰ synthesis and Phosphorylated extracellular signal- regulated kinase 1/2 (P-ERK1/2)expression in rat hepatic stellate cell (HSC) stimulated by platelet derived growth factor-BB (PDGF-BB), and to elucidate the molecular mechanism of Danshensu against hepatic fibrosis: Methods: Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl )-2,5-Diphennyltetrazolium bromide(MTT)colorimetric assay.Collagen type Ⅰ synthesis was detected by Immunocytochemical stain.Phosphorylated extracellular signal- regulated kinase 1/2 (P-ERK1/2) expression was detected by Western blot. Results:Danshensu could inhibit proliferation,collagen type Ⅰ synthesis and P-ERK1/2 expression of HSC stimulated by PDGF-BB. Conclusion:Danshensu can inhibit proliferation and Collagen type Ⅰ synthesis of HSC stimulated by PDGF-BB,the mechanism of which may be Danshensu, s inhibitory effect on ERK1/2 signal transduction pathway.
