CAJ | 학술논문

目的:观察糖皮质激素(以下简称激素)治疗对系统性红斑狼疮(SLE)患者Th1和Th2细胞因子表达的影响.方法:采用反转录-聚合酶链反虚(RT-PCR)法,检测SLE患者在激素治疗前、后,外周血单个核细胞(PBMC)中Th1类细胞因子γ干扰索(IFN-γ)和Th2类细胞因子白介素(IL)-4、IL-10 mRNA水平,并与正常人群比较.结果:激素治疗前SLE患者外周血内Th1和Th2类细胞凶子均高于正常人(P＜0.05),以Th2类细胞因子增高显著;治疗后两类细胞因子均明显下降(P＞0.05),但以Th1类细胞因子下降显著.结论:SLE患者存在T辅助细胞亚群失衡,激素治疗可部分纠正分泌失衡的细胞因子.
목적:관찰당피질격소(이하간칭격소)치료대계통성홍반랑창(SLE)환자Th1화Th2세포인자표체적영향.방법:채용반전록-취합매련반허(RT-PCR)법,검측SLE환자재격소치료전、후,외주혈단개핵세포(PBMC)중Th1류세포인자γ간우색(IFN-γ)화Th2류세포인자백개소(IL)-4、IL-10 mRNA수평,병여정상인군비교.결과:격소치료전SLE환자외주혈내Th1화Th2류세포흉자균고우정상인(P＜0.05),이Th2류세포인자증고현저;치료후량류세포인자균명현하강(P＞0.05),단이Th1류세포인자하강현저.결론:SLE환자존재T보조세포아군실형,격소치료가부분규정분비실형적세포인자.
Objective:To explore the effect of glucorticosteroids on the balance of Th1/Th2 cytokines in the patients with systemic lupus erythematosus(SEE).Methods:Thirty-eight patients with SLE enrolled in this study were all treated with glucorticosteroids.The mRNA levels of IFN-γ,IL-4 and IL-10 in the peripheral blood mononuclear ceHs(PBMCs)from the SLE patients before and after treatment were detected with reverse transcript polymerase chain reaction (RT-PER),which were compared with those in healthy controls. Results:The mRNA levels IFN-γ IL-4 and IL-10 in SLE patients before mRNA levels of IFN-γ,IL-4 and IL-10 in the SLE patients were all significantly reduced after the treatment(P＜0.05),with more decrease of IFN-γ.Conclusion:There Was an imbalance of Th1/Th2 cytokines in SLE patients,which may be partially rectified by glucorticosteroid therapy.