CAJ | 학술논문

目的探讨氨溴索诱导血红素加氧酶-1（HO-1）在胃内容物吸入性肺损伤中的抗炎作用机制。方法40只健康Sprague-Dawley大鼠随机分为4组：对照组、损伤组、氨溴索组、锌原卟啉(Znppix)组。采用蛋白免疫印迹法(Western blot)检测肺组织HO-1蛋白表达；肺泡灌洗液中炎性细胞计数，并使用ELISA法测定肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、IL-10含量；电镜下观察肺组织超微结构变化。结果氨溴索组HO-1蛋白表达灰度值较对照组及损伤组均升高（P值均＜0.05）。氨溴索组肺泡灌洗液中白细胞及中性粒细胞计数、TNF-α、IL-1β、IL-10较损伤组均降低(P值均＜0.05)，而Znppix组较氨溴索组均升高（P值均＜0.05）。电镜下氨溴索组肺组织超微结构较损伤组及Znppix组减轻。结论氨溴索可诱导HO-1表达，进一步通过抑制TNF-α、IL-1β、IL-10的产生在胃内容物吸入性肺损伤中发挥抗炎保护作用。
목적탐토안추색유도혈홍소가양매-1（HO-1）재위내용물흡입성폐손상중적항염작용궤제。방법40지건강Sprague-Dawley대서수궤분위4조：대조조、손상조、안추색조、자원계람(Znppix)조。채용단백면역인적법(Western blot)검측폐조직HO-1단백표체；폐포관세액중염성세포계수，병사용ELISA법측정폐포관세액중종류배사인자α(TNF-α)、백개소1β(IL-1β)、IL-10함량；전경하관찰폐조직초미결구변화。결과안추색조HO-1단백표체회도치교대조조급손상조균승고（P치균＜0.05）。안추색조폐포관세액중백세포급중성립세포계수、TNF-α、IL-1β、IL-10교손상조균강저(P치균＜0.05)，이Znppix조교안추색조균승고（P치균＜0.05）。전경하안추색조폐조직초미결구교손상조급Znppix조감경。결론 안추색가유도HO-1표체，진일보통과억제TNF-α、IL-1β、IL-10적산생재위내용물흡입성폐손상중발휘항염보호작용。
Objective To investigate the anti-inflammatory roles of heine oxygenase-1 (HO-1)induced by ambroxol in gastric aspiration lung injury. Methods Forty Sprague-Dawley rats were randomly divided into four groups:control group,injury group,ambroxol group and znppix group. The expression of HO-1 was examined by Western blot. Leucocyte and neutrophil leucocyte in bronchoalveolar iavage fluid were counted. Tumor necrosis factor-α (TNF-α), interleukin-1ββ (IL-1β) and IL-10 in bronchoalveolar lavage fluid were examined by enzyme linked immunosorbent assay. The ultrastructure of the pulmonary alveoli and its capillaries were studied by transmissional electromicroscopy. Results Compared with control group and injury group, the gray value of HO-1 in ambroxol group increased (all P ＜0.05).Compared with injury group, white blood cell and neutrophil leucocyte counts,TNF-α, IL-1β and IL-10 in bronchoalveolar lavage fluid of ambroxol group decreased ( all P ＜ 0.05 ). Compared with ambroxol group,the above indexes in znppix group increased (all P ＜0.05). Transmissional electromicroscopy showed that the pathological changes of ambroxol group were milder than those of injury group and znppix group. Conclusions Ambroxol may induc.e the expression of HO-1 and has anti-inflammatory effects by restraining the releasing of TNF-α, IL-1β, IL-10 in gastric aspiration lung injury.