国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2009年
1期
65-68
,共4页
核酸扩增技术%病原体检测%PCR-免疫层析技术%多重荧光实时PCR
覈痠擴增技術%病原體檢測%PCR-免疫層析技術%多重熒光實時PCR
핵산확증기술%병원체검측%PCR-면역층석기술%다중형광실시PCR
Nucleic acid amplification techniques%Pathogen detection%PCR-Immunochromatography test%Multiplex fluorescent real-time PCR
传统的病原体检测方法在检测敏感性、特异性、应用范围等方面存在许多不足.PCR技术已被用于感染早期的病原体检测,但在应用过程中也面临不少问题.近年来出现的新的核酸扩增技术可弥补普通PCR技术的缺点.此文就其中依赖核酸序列的扩增技术、PCR-胶体金免疫层析技术和多重荧光实时PcR技术进行综述.
傳統的病原體檢測方法在檢測敏感性、特異性、應用範圍等方麵存在許多不足.PCR技術已被用于感染早期的病原體檢測,但在應用過程中也麵臨不少問題.近年來齣現的新的覈痠擴增技術可瀰補普通PCR技術的缺點.此文就其中依賴覈痠序列的擴增技術、PCR-膠體金免疫層析技術和多重熒光實時PcR技術進行綜述.
전통적병원체검측방법재검측민감성、특이성、응용범위등방면존재허다불족.PCR기술이피용우감염조기적병원체검측,단재응용과정중야면림불소문제.근년래출현적신적핵산확증기술가미보보통PCR기술적결점.차문취기중의뢰핵산서렬적확증기술、PCR-효체금면역층석기술화다중형광실시PcR기술진행종술.
The traditional pathogen detection mathods have many disadvantages in the sensitivity, specificity and application range. There are still some problems in PCB, which has been used for pathogen detection in the early stage of infection. New nucleic acid amplification technologies may make up the deficiency of PCR. In this review, three methods including nucleic acid sequence-based amplification (NASBA), PCR-immunochromatography test and multiplex fluorescent real-time PCR technology are described.
