CAJ | 학술논문

目的探讨乙肝患者使用自体树突细胞和混合T淋巴细胞疫苗(简称抗HBV-DC-MTL)治疗慢性乙型肝炎的临床采血与回输的护理.方法取乙肝患者的肝素抗凝外周静脉血50 ml,通过实验室生物免疫技术培养获得成熟的树突细胞和混合T淋巴细胞疫苗,培养第7天将收获的DC、第14天收获的抗HBV-MTL分别回输给患者.并对38例乙肝患者采集细胞与回输的护理进行观察.结果 38例患者好转36例,无效1例,治愈1例.结论采用肝素抗凝的50 ml注射器加9号输液头皮针在大血管处采血,采血成功率高；标本4℃左右冷藏保存,2h内分离培养,是收获细胞疫苗成功的关键；回输的方法,安全度高,并发症少.
목적 탐토을간환자사용자체수돌세포화혼합T림파세포역묘(간칭항HBV-DC-MTL)치료만성을형간염적림상채혈여회수적호리.방법 취을간환자적간소항응외주정맥혈50 ml,통과실험실생물면역기술배양획득성숙적수돌세포화혼합T림파세포역묘,배양제7천장수획적DC、제14천수획적항HBV-MTL분별회수급환자.병대38례을간환자채집세포여회수적호리진행관찰.결과 38례환자호전36례,무효1례,치유1례.결론 채용간소항응적50 ml주사기가9호수액두피침재대혈관처채혈,채혈성공솔고；표본4℃좌우랭장보존,2h내분리배양,시수획세포역묘성공적관건；회수적방법,안전도고,병발증소.
Objective To explore the clinical blood collection and doping nursing care for hepatitis B patients who use self dendritic cells (DC)and mixed T lymphocytes vaccine (resistant HBV-DC-MTL for short ) for treatment.Methods Thirty-eight chronic hepatitis B virus (HBV) infection patients including 33 chronic hepatitis B and 5 chronic HBV carriers undergone clinical trial during December 2010 to July 2011.Fifty millilitre heparin anticoagulation peripheral venous blood was taken for cultivating mature DC and mixed T lymphocytes vaccine with laboratory biological immune technology,the DC obtained on the seventh day and resistant HBV-MTL obtained on the fourteenth day intravenously injected to the patients and we observed the cell collection and doping nursing effect.Results No case was recollected blood for blood coagulation,the obtained cells after cultivated were returned to the patient without adverse effects and showed a distinct effect in 38 patients.Conclusions It is more successful in blood collection by using the heparin 50 ml syringe and anticoagulant 9 infusion scalp needle in the great vessels; it is the key in obtaining cell vaccine that the specimen is cold preservation in 4 ℃,and separated and cultured in 2 h; doping method is of high safety and Iess complications.