中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2011年
12期
1255-1258
,共4页
曲梅%张新%刘桂荣%黄瑛%李洁%李锡太%刘元%严寒秋%刘白微%黎新宇%王全意%黄芳
麯梅%張新%劉桂榮%黃瑛%李潔%李錫太%劉元%嚴寒鞦%劉白微%黎新宇%王全意%黃芳
곡매%장신%류계영%황영%리길%리석태%류원%엄한추%류백미%려신우%왕전의%황방
副溶血弧菌%病原学%分子分型
副溶血弧菌%病原學%分子分型
부용혈호균%병원학%분자분형
Vibrio parahaemolyticus%Etiology%Molecular typing
目的 了解北京市副溶血弧菌腹泻病例分离株的病原学和分子流行病学特征.方法 2010年4-12月从临床收集散发腹泻病例2118份粪便标本,进行副溶血弧菌的分离培养、生化鉴定.阳性菌株进行血清分型；采用药敏纸片法对12种抗生素进行敏感性检测；用实时荧光定量PCR方法检测毒力基因tlh、tdh和trh；用脉冲场凝胶电泳(PFGE)进行分子分型.结果 2118份粪便标本分离出副溶血弧菌114株,阳性分离率为5.38％.114株副溶血弧菌分属于23种血清型,其中O3:K6为优势血清型,占63.16％.临床分离株对氨苄西林和庆大霉素产生耐药；对阿莫西林、头孢曲松、氯霉素、亚胺培南、萘啶酸和四环素等均具有较高敏感性.全部菌株tlh基因阳性；tdh存在于大部分菌株中,所占比例为93.86％；只有1株菌trh阳性.O3:K6型菌株tdh基因阳性率(98.61％)明显高于非O3:K6型菌株(85.71％)(P=0.0098).114株副溶血弧菌分成54种PFGE型别,72株O3:K6型菌株分成34种PFGE型别,带型分散,无明显聚集性.结论 北京地区感染性腹泻病例副溶血弧菌分离株以O3:K6型为主.毒力基因tlh和tdh携带率高,且O3:K6型临床分离株毒力更强.副溶血弧菌对多数抗生素均具有较高敏感性.PFGE结果提示北京地区流行的副溶血弧菌存在多克隆来源.
目的 瞭解北京市副溶血弧菌腹瀉病例分離株的病原學和分子流行病學特徵.方法 2010年4-12月從臨床收集散髮腹瀉病例2118份糞便標本,進行副溶血弧菌的分離培養、生化鑒定.暘性菌株進行血清分型；採用藥敏紙片法對12種抗生素進行敏感性檢測；用實時熒光定量PCR方法檢測毒力基因tlh、tdh和trh；用脈遲場凝膠電泳(PFGE)進行分子分型.結果 2118份糞便標本分離齣副溶血弧菌114株,暘性分離率為5.38％.114株副溶血弧菌分屬于23種血清型,其中O3:K6為優勢血清型,佔63.16％.臨床分離株對氨芐西林和慶大黴素產生耐藥；對阿莫西林、頭孢麯鬆、氯黴素、亞胺培南、萘啶痠和四環素等均具有較高敏感性.全部菌株tlh基因暘性；tdh存在于大部分菌株中,所佔比例為93.86％；隻有1株菌trh暘性.O3:K6型菌株tdh基因暘性率(98.61％)明顯高于非O3:K6型菌株(85.71％)(P=0.0098).114株副溶血弧菌分成54種PFGE型彆,72株O3:K6型菌株分成34種PFGE型彆,帶型分散,無明顯聚集性.結論 北京地區感染性腹瀉病例副溶血弧菌分離株以O3:K6型為主.毒力基因tlh和tdh攜帶率高,且O3:K6型臨床分離株毒力更彊.副溶血弧菌對多數抗生素均具有較高敏感性.PFGE結果提示北京地區流行的副溶血弧菌存在多剋隆來源.
목적 료해북경시부용혈호균복사병례분리주적병원학화분자류행병학특정.방법 2010년4-12월종림상수집산발복사병례2118빈분편표본,진행부용혈호균적분리배양、생화감정.양성균주진행혈청분형；채용약민지편법대12충항생소진행민감성검측；용실시형광정량PCR방법검측독력기인tlh、tdh화trh；용맥충장응효전영(PFGE)진행분자분형.결과 2118빈분편표본분리출부용혈호균114주,양성분리솔위5.38％.114주부용혈호균분속우23충혈청형,기중O3:K6위우세혈청형,점63.16％.림상분리주대안변서림화경대매소산생내약；대아막서림、두포곡송、록매소、아알배남、내정산화사배소등균구유교고민감성.전부균주tlh기인양성；tdh존재우대부분균주중,소점비례위93.86％；지유1주균trh양성.O3:K6형균주tdh기인양성솔(98.61％)명현고우비O3:K6형균주(85.71％)(P=0.0098).114주부용혈호균분성54충PFGE형별,72주O3:K6형균주분성34충PFGE형별,대형분산,무명현취집성.결론 북경지구감염성복사병례부용혈호균분리주이O3:K6형위주.독력기인tlh화tdh휴대솔고,차O3:K6형림상분리주독력경강.부용혈호균대다수항생소균구유교고민감성.PFGE결과제시북경지구류행적부용혈호균존재다극륭래원.
Objective To understand the etiological and molecular-epiderniological characteristics of Vibrio parahaemolyticus in Beijing.Methods Stool specimens from sporadic diarrheal patients were collected during April to December,2010.Culture and serotyping were used to detect the Vibrio parahaemolyticus from the 2118 specimens.All the positive strains were tested for drug sensitivity by Kirby-Bauer method.Real-time PCR was used to detect the existence of three virulence genes tlh,tdh and trh.Molecular typing on Vibrio parahaerrolyticus isolates was completed by pulsed field gel electrophoresis(PFGE).Results 114 out of the 2118 specimens were Vibrio parahaemolyticus positive,with the positive rate as 5.38％.114 isolates belonged to 23 serotypes,with the dominant (63.16％)serotype as O3:K6.Strains isolated from clinical manifestation patients were resistant to antibiotics-ampicillin and gentamicin,while with high sensitivity to amoxicillin,ceftriaxone,chloromycetin,imipenem,nalidixic acid and tetracycline.Virulence gene detection was positive to tlh for all the strains,but most to tdh,while only one strain to trh.The positive rate oftdh among O3:K6 strains(98.61％)was higher than that in those non-O3:K6 strains(85.71％)(P=0.0098).114 isolates were discriminated into 54 different PFGE patterns,while 72 O3:K6 strains into 34 patterns without the clustering characteristic.Conclusion Vibrio parahaemolyticus strains isolated from diarrheal patients in Beijing were dominated by O3:K6 strains,with stronger virulence.The positive rates of genes tlh and tdh were high.Vibrio parahaemolyticus strains showed high sensitivity to most antibiotics.The prevalent Vibrio parahaemolyticus strains in Beijing had various sources of clones.
