CAJ | 학술논문

目的运用基因芯片技术研究抗环瓜氨酸肽(CCP)抗体阳性和阴性类风湿关节炎(RA)患者外周血单个核细胞(PBMC)基因表达谱差异,探索差异存在的基因表达基础.方法提取各5例抗CCP抗体阳性、阴性RA患者、健康人PBMC中总RNA,运用Ⅲumina基因表达谱芯片分析每个样本47231个基因变化.结果与健康对照组相比,抗CCP抗体阳性和阴性RA患者有88个差异表达基因,其中上调表达基因51个,下调表达基因37个,这些差异表达基因主要涉及细胞因子、细胞凋亡、细胞周期、细胞因子、信号转导、趋化因子等.抗CCP抗体阳性和阴性RA患者之间有20个基因表达差异有统计学意义,其中上调表达基因9个,下调表达基因11个,这些差异表达基因主要涉及传递蛋白、转录调控、信号转导、代谢、细胞周期等.结论抗CCP抗体阳性和阴性RA患者之间存在差异表达基因,筛选到的差异基因如杀伤细胞免疫球蛋白受体基因、干扰素诱导基因、几丁质酶家族成员CHI3L1等为进一步研究RA发病机制及发现新治疗靶标提供重要线索.
목적 운용기인심편기술연구항배과안산태(CCP)항체양성화음성류풍습관절염(RA)환자외주혈단개핵세포(PBMC)기인표체보차이,탐색차이존재적기인표체기출.방법 제취각5례항CCP항체양성、음성RA환자、건강인PBMC중총RNA,운용Ⅲumina기인표체보심편분석매개양본47231개기인변화.결과 여건강대조조상비,항CCP항체양성화음성RA환자유88개차이표체기인,기중상조표체기인51개,하조표체기인37개,저사차이표체기인주요섭급세포인자、세포조망、세포주기、세포인자、신호전도、추화인자등.항CCP항체양성화음성RA환자지간유20개기인표체차이유통계학의의,기중상조표체기인9개,하조표체기인11개,저사차이표체기인주요섭급전체단백、전록조공、신호전도、대사、세포주기등.결론 항CCP항체양성화음성RA환자지간존재차이표체기인,사선도적차이기인여살상세포면역구단백수체기인、간우소유도기인、궤정질매가족성원CHI3L1등위진일보연구RA발병궤제급발현신치료파표제공중요선색.
Objective To explore the differences of gene expression of peripheral blood mononuclear cell (PBMC) between the anti-cyclic citrullinated peptide antibody (ACCPA) positive and ACCPA negative patients with rheumatoid arthritis (RA) by microarray analysis.Methods Total RNA was extracted from PBMC of 5 ACCPA positive and ACCPA negative patients with RA,and age-and sex-matched control subjects respectively.Ⅲumina oligonucleotide microarray was used to characterize 47231gene expression profile for each sample.Results Among the target genes,88 differentially expressed genes were identified in RA patients.Fifty-one up-regulated genes and 37 down-regulated genes were found in RA patients compared to those in control subjects (fold change＞1.5).The differential expression of genes were associated with apoptosis,cytokine,signal identification protein,chemotaxis factor etc.There were 20 differential expression genes between the ACCPA positive and ACCPA negative patients with RA,9 up-regulated genes and 11 downregulated genes were found.The differential expression genes were associated with protein biding,,translation control,signal identification protein,cell cycle,metabolism etc.Conclusion There are differential gene expression between the ACCPA positive and ACCPA negative patients with RA.Genes screened from the target such as IFI,KIR,CHI3L1 can provi-de important information for further study and treatment of RA.