中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1131-1133
,共3页
王妙淑%王卫军%张近波%方钱%李剑锋%金鹏飞%侯建毅%李智涛
王妙淑%王衛軍%張近波%方錢%李劍鋒%金鵬飛%侯建毅%李智濤
왕묘숙%왕위군%장근파%방전%리검봉%금붕비%후건의%리지도
急性重症胰腺炎%细胞间黏附分子-1%参麦注射液
急性重癥胰腺炎%細胞間黏附分子-1%參麥註射液
급성중증이선염%세포간점부분자-1%삼맥주사액
Severe acute pancreatitis%Intercellular adhesion molecule-1%Shenmai injection
目的 观察参麦注射液对重症急性胰腺炎(SAP)大鼠模型血中可溶性细胞间黏附分子-1(sICAM-1)水平及胰腺组织中1CAM-1 mRNA的影响.方法 将108只成年雄性SD大鼠随机分为3组:对照组(A组)、SAP模型组(B组)及参麦治疗组(C组)；其中B组及C组再分为4、8、16、24h4个亚组,各个亚组及A组各12只.对各组大鼠血清中sICAM-1水平及胰腺组织中ICAM-1mRNA水平进行比较.结果 B组各时间点大鼠血清sICAM-1浓度较A组均显著升高(128.07±15.86比87.31±13.16、142.33±22.61比98.82±11.39、187.45±17.39比129.51±15.61、176.18±19.36比123.57±17.03)(P＜0.05)；C组各时间点大鼠血清sICAM-1浓度较B组均显著降低(92.55±8.09比128.07±15.86、105.28±14.29比142.33±22.61、142.36±18.25比187.45±17.39、131.32±21.83比176.18±19.36)(P＜0.05)；B组各时间点大鼠胰腺组织内ICAM-1 mRNA表达水平较A组均显著升高(0.97±0.12比0.52±0.11、1.08±0.18比0.61±0.13、1.32±0.18比0.82±0.14、1.23±0.09比0.74±0.13)(P＜0.05)；C组各时间点大鼠胰腺组织内ICAM-1 mRNA表达水平较B组均显著降低(0.59±0.13比0.97±0.12、0.68±0.09比1.08±0.18、0.96±0.20比1.32±0.18、0.88±0.09比1.23±0.09)(P＜0.05).结论 参麦注射液通过下调SAP大鼠ICAM-1 mRNA及ICAM-1的表达,起到改善SAP大鼠微循环、保护SAP大鼠的作用.
目的 觀察參麥註射液對重癥急性胰腺炎(SAP)大鼠模型血中可溶性細胞間黏附分子-1(sICAM-1)水平及胰腺組織中1CAM-1 mRNA的影響.方法 將108隻成年雄性SD大鼠隨機分為3組:對照組(A組)、SAP模型組(B組)及參麥治療組(C組)；其中B組及C組再分為4、8、16、24h4箇亞組,各箇亞組及A組各12隻.對各組大鼠血清中sICAM-1水平及胰腺組織中ICAM-1mRNA水平進行比較.結果 B組各時間點大鼠血清sICAM-1濃度較A組均顯著升高(128.07±15.86比87.31±13.16、142.33±22.61比98.82±11.39、187.45±17.39比129.51±15.61、176.18±19.36比123.57±17.03)(P＜0.05)；C組各時間點大鼠血清sICAM-1濃度較B組均顯著降低(92.55±8.09比128.07±15.86、105.28±14.29比142.33±22.61、142.36±18.25比187.45±17.39、131.32±21.83比176.18±19.36)(P＜0.05)；B組各時間點大鼠胰腺組織內ICAM-1 mRNA錶達水平較A組均顯著升高(0.97±0.12比0.52±0.11、1.08±0.18比0.61±0.13、1.32±0.18比0.82±0.14、1.23±0.09比0.74±0.13)(P＜0.05)；C組各時間點大鼠胰腺組織內ICAM-1 mRNA錶達水平較B組均顯著降低(0.59±0.13比0.97±0.12、0.68±0.09比1.08±0.18、0.96±0.20比1.32±0.18、0.88±0.09比1.23±0.09)(P＜0.05).結論 參麥註射液通過下調SAP大鼠ICAM-1 mRNA及ICAM-1的錶達,起到改善SAP大鼠微循環、保護SAP大鼠的作用.
목적 관찰삼맥주사액대중증급성이선염(SAP)대서모형혈중가용성세포간점부분자-1(sICAM-1)수평급이선조직중1CAM-1 mRNA적영향.방법 장108지성년웅성SD대서수궤분위3조:대조조(A조)、SAP모형조(B조)급삼맥치료조(C조)；기중B조급C조재분위4、8、16、24h4개아조,각개아조급A조각12지.대각조대서혈청중sICAM-1수평급이선조직중ICAM-1mRNA수평진행비교.결과 B조각시간점대서혈청sICAM-1농도교A조균현저승고(128.07±15.86비87.31±13.16、142.33±22.61비98.82±11.39、187.45±17.39비129.51±15.61、176.18±19.36비123.57±17.03)(P＜0.05)；C조각시간점대서혈청sICAM-1농도교B조균현저강저(92.55±8.09비128.07±15.86、105.28±14.29비142.33±22.61、142.36±18.25비187.45±17.39、131.32±21.83비176.18±19.36)(P＜0.05)；B조각시간점대서이선조직내ICAM-1 mRNA표체수평교A조균현저승고(0.97±0.12비0.52±0.11、1.08±0.18비0.61±0.13、1.32±0.18비0.82±0.14、1.23±0.09비0.74±0.13)(P＜0.05)；C조각시간점대서이선조직내ICAM-1 mRNA표체수평교B조균현저강저(0.59±0.13비0.97±0.12、0.68±0.09비1.08±0.18、0.96±0.20비1.32±0.18、0.88±0.09비1.23±0.09)(P＜0.05).결론 삼맥주사액통과하조SAP대서ICAM-1 mRNA급ICAM-1적표체,기도개선SAP대서미순배、보호SAP대서적작용.
Objective To explore the impact of Shenmai injection on the levels of plasma soluble intercellular adhesion molecule-1 ( sICAM-1 ) and ICAM-1 mRNA in the pancreas tissue of rats with severe acute pancreatitis (SAP).Methods One hundred and eight SD male rats were randomly divided into three groups:sham operation group (group A),SAP model group (group B),Shenmai injection group ( group C).The rats in groups B and C were further divided into four subgroups based on the time points of constructing model:4,8,16 and 24 h subgroups (n =12 each).The levels of plasma sICAM-1 and the ICAM-1 mRNA in the pancreas tissue of rats were compared among the three groups.Results The levels of plasma sICAM-1 were significantly higher in group B than in 4,8,16 and 24 h subgroups of group A (128.07±15.86 vs 87.31 ±13.16,142.33±22.61 vs 98.82 ±11.39,187.45±17.39 vs 129.51 ±15.61,and 176.18 ± 19.36 vs 123.57 ± 17.03) ( all P ＜0.05).The levels of plasma sICAM-1 were significantly lower in group C than those in 4,8,16 and 24 h subgroups of group B (92.55 ± 8.09 vs 128.07 ± 15.86,105.28 ± 14.29 vs 142.33 ± 22.61,142.36 ± 18.25 vs 187.45 ± 17.39,131.32 ± 21.83 vs 176.18 ±19.36) ( all P ＜ 0.05 ).The expression of 1CAM-1 mRNA in the pancreatic tissue was significantly higher in group B than that in4,8,16 and 24 h subgroups of group A (0.97 ±0.12 vs 0.52 ±0.11,1.08 ±0.18 vs 0.61±0.13,1.32±0.18 vs0.82±0.14,1.23 ±0.09vs0.74±0.13) (allP＜0.05).The expression of ICAM-1 mRNA in the pancreatic tissue was significantly lower in group C than that in 4,8,16 and 24 h subgroups of group B ( 0.59 ± 0.13 vs 0.97 ± 0.12,0.68 ± 0.09 vs 1.08 ± 0.18,0.96 ±0.20vs1.32±0.18,0.88±0.09vs1.23±0.09) (allP＜0.05).Conclusion Shenmai injection could improve microcirculation and protect rats with SAP through decreasing the levels of plasma sICAM-1and the ICAM-1 mRNA of the pancreas tissue.
