CAJ | 학술논문

硫醇是生物体中许多蛋白质和小分子的重要组成部分,在细胞的抗氧化系统中具有重要的作用,定量检测硫醇在生物化学和临床化学中具有重要的意义.荧光法由于其具有灵敏度高、能够实现对活体甚至单个细胞的实时可视化示踪的优点,成为目前广泛采用检测细胞内硫醇类物质的一种重要手段.本文引用文献51篇,按荧光探针与巯基作用机理的不同分类,就近年来该领域的研究进展做了比较系统的评述,并展望了此类探针的发展趋势和应用前景.
류순시생물체중허다단백질화소분자적중요조성부분,재세포적항양화계통중구유중요적작용,정량검측류순재생물화학화림상화학중구유중요적의의.형광법유우기구유령민도고、능구실현대활체심지단개세포적실시가시화시종적우점,성위목전엄범채용검측세포내류순류물질적일충중요수단.본문인용문헌51편,안형광탐침여구기작용궤리적불동분류,취근년래해영역적연구진전주료비교계통적평술,병전망료차류탐침적발전추세화응용전경.
Thiols, which are components of many proteins and simple molecules, play an important role in the cellular antioxidant defense system. The quantitative determination of thiols is important in biochemistry and clinical chemistry. Fluorescent probes, which have its apparent advantages in sensitivity and, most importantly, in imaging thiols in vivo, even in single living cells, appear to be particularly attractive. In this review, we classify the fluorescent probes based on their different reaction mechanisms with thiols and summarize the recent progresses of thiols fluorescent probes with fifty-one