海洋科学
海洋科學
해양과학
MARINE SCIENCES
2009年
11期
11-14
,共4页
刘松%邢荣娥%蔡圣宝%于华华%李鹏程
劉鬆%邢榮娥%蔡聖寶%于華華%李鵬程
류송%형영아%채골보%우화화%리붕정
壳聚糖%壳聚糖硫酸酯金属配合物%超氧阴离子自由基(O2·-)%羟自由基(·OH)%抗氧化活性
殼聚糖%殼聚糖硫痠酯金屬配閤物%超氧陰離子自由基(O2·-)%羥自由基(·OH)%抗氧化活性
각취당%각취당류산지금속배합물%초양음리자자유기(O2·-)%간자유기(·OH)%항양화활성
chitosan%the complexes of sulfation chitosan with metalion%superoxide radical%hydroxyl radical%antioxidant activity
以吩嗪硫酸甲酯-NADH为超氧阴离子自由基(O_2~(·-))产生、检测体系和EDTANa_2-Fe(Ⅱ)-H_2O_2为羟自由基(~·OH)产生、检测体系,对壳聚糖硫酸酯铜、锌配合物和不同分子质量壳聚糖进行了抗O_2~(·-)和~·OH自由基的活性研究.结果表明,壳聚糖硫酸酯金属配合物对于O_2~(·-)自由基的清除能力明显高于壳聚糖,在质量浓度为0.025 g/L时,壳聚糖硫酸酯铜配合物对O_2~(·-)自由基的清除能力达到94.18%,壳聚糖硫酸酯锌配合物达到93.19%;壳聚糖硫酸酯铜、锌配合物对~·OH自由基的清除能力(67.39%、60.46%)低于相同分子质量的壳聚糖(88.06%),而高于高分子质量壳聚糖761 ku(18.71%);壳聚糖分子质量大小对O_2~(·-)和~·OH自由基的清除能力有较大影响,质量浓度为1.6 g/L壳聚糖分子质量为20 ku时,对O_2~(·-)清除率达54.69%,而分子质量在761 ku时,对O_2~(·-)清除率仅为35.50%;各样品对O_2~(·-)和~·OH自由基的清除能力均随着质量浓度的增加而上升,壳聚糖硫酸酯铜、锌配合物在相当低的浓度下(0.025 g/L)就可以达到明显清除O_2~(·-)自由基的效果(≥90%).
以吩嗪硫痠甲酯-NADH為超氧陰離子自由基(O_2~(·-))產生、檢測體繫和EDTANa_2-Fe(Ⅱ)-H_2O_2為羥自由基(~·OH)產生、檢測體繫,對殼聚糖硫痠酯銅、鋅配閤物和不同分子質量殼聚糖進行瞭抗O_2~(·-)和~·OH自由基的活性研究.結果錶明,殼聚糖硫痠酯金屬配閤物對于O_2~(·-)自由基的清除能力明顯高于殼聚糖,在質量濃度為0.025 g/L時,殼聚糖硫痠酯銅配閤物對O_2~(·-)自由基的清除能力達到94.18%,殼聚糖硫痠酯鋅配閤物達到93.19%;殼聚糖硫痠酯銅、鋅配閤物對~·OH自由基的清除能力(67.39%、60.46%)低于相同分子質量的殼聚糖(88.06%),而高于高分子質量殼聚糖761 ku(18.71%);殼聚糖分子質量大小對O_2~(·-)和~·OH自由基的清除能力有較大影響,質量濃度為1.6 g/L殼聚糖分子質量為20 ku時,對O_2~(·-)清除率達54.69%,而分子質量在761 ku時,對O_2~(·-)清除率僅為35.50%;各樣品對O_2~(·-)和~·OH自由基的清除能力均隨著質量濃度的增加而上升,殼聚糖硫痠酯銅、鋅配閤物在相噹低的濃度下(0.025 g/L)就可以達到明顯清除O_2~(·-)自由基的效果(≥90%).
이분진류산갑지-NADH위초양음리자자유기(O_2~(·-))산생、검측체계화EDTANa_2-Fe(Ⅱ)-H_2O_2위간자유기(~·OH)산생、검측체계,대각취당류산지동、자배합물화불동분자질량각취당진행료항O_2~(·-)화~·OH자유기적활성연구.결과표명,각취당류산지금속배합물대우O_2~(·-)자유기적청제능력명현고우각취당,재질량농도위0.025 g/L시,각취당류산지동배합물대O_2~(·-)자유기적청제능력체도94.18%,각취당류산지자배합물체도93.19%;각취당류산지동、자배합물대~·OH자유기적청제능력(67.39%、60.46%)저우상동분자질량적각취당(88.06%),이고우고분자질량각취당761 ku(18.71%);각취당분자질량대소대O_2~(·-)화~·OH자유기적청제능력유교대영향,질량농도위1.6 g/L각취당분자질량위20 ku시,대O_2~(·-)청제솔체54.69%,이분자질량재761 ku시,대O_2~(·-)청제솔부위35.50%;각양품대O_2~(·-)화~·OH자유기적청제능력균수착질량농도적증가이상승,각취당류산지동、자배합물재상당저적농도하(0.025 g/L)취가이체도명현청제O_2~(·-)자유기적효과(≥90%).
The O_2~(·-)and ~·OH scavenging activities of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)and DMW chitosan were investigated.Colorimetric method was used,where phenzine methosulphate(PMS)/dinucleotide-reduced(NADH)and Fe/H_2O_2 were used as producing reagents and Nitro blue tetrazolium (NBT) and Fanhonghua as detecting reagents for O_2~(·-)and ~·OH.As expected,we obtained several satisfying resulte, as follow:
Firstly,compared with DMW chitosan,the complexes of sulfation chitosan with copper(Ⅱ)or zinc (Ⅱ)had the stronger inhibition effect on O_2~(·-).For example,when mass concentration of samples was 0.025 g/L,the O_2~(·-)scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)and the complexes of sulfation chitosan with zinc(Ⅱ)were 94.18%and 93.19%respectively.Secondly,scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)(67.39%,60.46%,3.2 g/L)on ~·OH was between low molecular weight chitosan(20 ku,88.06%,3.2 g/L)and high molecular weight chitosan(761 ku,18.71%,3.2 g/L).Thirdly,low molecular weight chitosan had stronger scavenging effect
on O_2~(·-)and ~·OH than high molecular weight chitosan.At 1.6 g/L,the scavenging activity of low molecular weight chitosan(20 ku)on O_2~(·-)reached 54.69%,while that of high molecular weight chitosan(761 ku)was 35.50%.Finally,the scavenging activity of several samples increased with increase in their mass concentration.However,the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)had a quite strong scavenging action to O_2~(·-),their scavenging activity barely had obvious changing over 0.025 g/L.This result also showed that the complexes of sulfation chitosan with metalion were very effective antioxidant.
