CAJ | 학술논문

目的通过观察视网膜内源性脑源性神经营养因:F(BDNF)表达的变化,探讨玻璃体注射携带人脑源性神经营养因子(hBDNF)的重组腺伴随病毒(rAAV-BDNF)对急性高眼压兔眼神经损害的保护机制.方法 24只健康日本大耳白兔任选一眼作为造模眼(为模型组,共24眼),用生理盐水前房灌注法造成急性高眼压模型,对侧眼不作任何处理作为正常对照组(24眼).另24只健康日本大耳白兔任选一眼作为造模眼(为BDNF组,共24眼),BDNF组在造模前3d玻璃体内注射10μl rAAV-BDNF.于造模后第1、3、7、14d三组各摘除6只观察眼做病理切片,进行免疫组化染色,观察视网膜内源性BDNF表达.结果模型组兔眼视网膜内源性BDNF表达阳性细胞数减少,BDNF组兔眼视网膜内源性BDNF表达阳性细胞数较对照组及模型组增加(P<0.05,P<0.01).结论 rAAV-BDNF基因转染通过增加视网膜内源性BDNF的表达起到神经保护作用.玻璃体注射是rAAV-BDNF转染视网膜的有效途径.
목적 통과관찰시망막내원성뇌원성신경영양인:F(BDNF)표체적변화,탐토파리체주사휴대인뇌원성신경영양인자(hBDNF)적중조선반수병독(rAAV-BDNF)대급성고안압토안신경손해적보호궤제.방법 24지건강일본대이백토임선일안작위조모안(위모형조,공24안),용생리염수전방관주법조성급성고안압모형,대측안불작임하처리작위정상대조조(24안).령24지건강일본대이백토임선일안작위조모안(위BDNF조,공24안),BDNF조재조모전3d파리체내주사10μl rAAV-BDNF.우조모후제1、3、7、14d삼조각적제6지관찰안주병리절편,진행면역조화염색,관찰시망막내원성BDNF표체.결과 모형조토안시망막내원성BDNF표체양성세포수감소,BDNF조토안시망막내원성BDNF표체양성세포수교대조조급모형조증가(P<0.05,P<0.01).결론 rAAV-BDNF기인전염통과증가시망막내원성BDNF적표체기도신경보호작용.파리체주사시rAAV-BDNF전염시망막적유효도경.
Objective To observe the changes in the expression of brain derived neurotrophic factor (BDNF) gene in the retina of rabbits with acute high intraocular pressure (IOP) after injection of recombinant adeno-associated virus (rAAV) vector containing human BDNF gene (rAAV-hBDNF), and investigate the neuroprotective mechanism of rAAV-hBDNF. Methods The unilateral eyes of 24 white rabbits were randomly chosen as the model group with high IOP induced by saline perfusion into the anterior chamber, and the contralateral eyes served as the control group without treatment. In another 24 white rabbits, 10 μl rAAV-BDNF was injected into the vitreous body of one of the eyes 3 days before induction of high IOP. On days 1,3,7, and 14 after perfusion, the bilateral eyes of 6 rabbits were excised for immunohistochemistry for the expression of endogenous BDNF gene in the retina. Results The number of BDNF-positive cells in the retina decreased after induction of high IOP, and injection of rAAV-hBDNF resulted in a significant increase in BDNF-positive cells as compared with the positive cell number in the high IOP model and control groups (P<0.05, P<0.01). Conclusion rAAV-mediated BDNF gene transfection can increase endogenous BDNF expression in the retina of rabbits with acute high IOP. Intravitreous injection is an effective pathway for rAAV-hBDNF gene transfection into the retina.