CAJ | 학술논문

研究了非抑制型阳离子交换色谱中色谱柱温度(25 ～50 ℃)对碱金属离子(Li~+、Na~+、K~+、Rb~+ )和碱土金属离子(Mg~(2+)、Ca~(2+)、Sr~(2+) )以及NH+4 保留的影响.在 Shim-pack IC-C1磺酸型阳离子交换柱上,以硝酸为流动相分离碱金属离子,以乙二胺或乙二胺-草酸(柠檬酸)为流动相分离碱土金属离子,随着色谱柱温度的升高,碱金属和碱土金属离子的保留时间均增长,其范特霍夫曲线具有良好的线性关系,斜率为负值,表明在此条件下碱金属和碱土金属离子的保留为吸热过程.在Shim-pack IC-SC1羧酸型阳离子交换柱上,以硫酸为流动相同时分离碱金属和碱土金属离子,随着色谱柱温度的升高,Mg~(2+)、Ca~(2+) 的保留时间增长,而 K~+、Rb~+ 的保留时间缩短,Li~+、Na~+、NH+4 的保留时间基本不变.在此条件下,Mg~(2+)、Ca~(2+)、K~+和Rb~+的范特霍夫曲线具有良好的线性关系,其中Mg~(2+)和Ca~(2+) 的曲线斜率为负值,K~+和Rb~+的曲线斜率为正值,表明Mg~(2+)和Ca~(2+) 的保留表现为吸热过程,K~+和Rb~+的保留表现为放热过程.研究表明在不同固定相和流动相条件下,色谱柱温度对碱金属和碱土金属离子保留行为的影响不同.
연구료비억제형양리자교환색보중색보주온도(25 ～50 ℃)대감금속리자(Li~+、Na~+、K~+、Rb~+ )화감토금속리자(Mg~(2+)、Ca~(2+)、Sr~(2+) )이급NH+4 보류적영향.재 Shim-pack IC-C1광산형양리자교환주상,이초산위류동상분리감금속리자,이을이알혹을이알-초산(저몽산)위류동상분리감토금속리자,수착색보주온도적승고,감금속화감토금속리자적보류시간균증장,기범특곽부곡선구유량호적선성관계,사솔위부치,표명재차조건하감금속화감토금속리자적보류위흡열과정.재Shim-pack IC-SC1최산형양리자교환주상,이류산위류동상동시분리감금속화감토금속리자,수착색보주온도적승고,Mg~(2+)、Ca~(2+) 적보류시간증장,이 K~+、Rb~+ 적보류시간축단,Li~+、Na~+、NH+4 적보류시간기본불변.재차조건하,Mg~(2+)、Ca~(2+)、K~+화Rb~+적범특곽부곡선구유량호적선성관계,기중Mg~(2+)화Ca~(2+) 적곡선사솔위부치,K~+화Rb~+적곡선사솔위정치,표명Mg~(2+)화Ca~(2+) 적보류표현위흡열과정,K~+화Rb~+적보류표현위방열과정.연구표명재불동고정상화류동상조건하,색보주온도대감금속화감토금속리자보류행위적영향불동.
The effects of column temperatures varying from 25 ℃ to 50 ℃ on the retention times of alkali metal ions(Li~+,Na~+,K~+,Rb~+),alkaline earth metal ions(Mg~(2+),Ca~(2+),Sr~(2+)) and ammonium ion(NH+4) were studied by non-suppressed cation-exchange ion chromatography.When nitric acid was used as mobile phase for separating alkali metal ions and ethylenediamine or ethylenediamine-oxalic acid as mobile phase for separating alkaline earth metals ions on Shim-pack IC-C1 of sulfonic cation-exchange column,the retention times of alkali metals and alkaline earth metal ions increased with the increase of column temperatures.The Van't Hoff plots for those ions were all linear with negative slopes indicating that the retention was of an endothermic process.When alkali metal and alkaline earth metal ions were simultaneously separated using sulfuric acid as mobile phase on Shim-pack IC-SC1 of carboxylic acid cation-exchange column,the retention times of Mg~(2+) and Ca~(2+) increased and K~+ and Rb~+ decreased with the increase of column temperatures,but those of Li~+,Na~+ and NH+4 ions varied insignificantly.The Van't Hoff plots for Mg~(2+) and Ca~(2+) were linear with negative slopes and those for K~+ and Rb~+ were linear with positive slopes indicating that the retention for Mg~(2+) and Ca~(2+) was of an endothermic process and that for K~+ and Rb~+was an exothermic one.The results showed that the effects of column temperatures on the retentions of alkali metal and alkaline earth metal ions varied with different stationary phases and mobile phases.