CAJ | 학술논문

丙二醛(Malondialdehyde, MDA)是机体内普遍存在的活性羰基化合物代表成分之一,能够与蛋白质发生羰-氨交联反应,生成老年色素荧光物质.本文探讨儿茶素单体成分对MDA应激引起的蛋白质类老年色素荧光物质生成体系的影响,并对其构效关系进行初步分析.结果表明:儿茶素能显著抑制MDA诱导的蛋白质类老年色素物质生成,且酯型儿茶素活性较强,抑制作用除与直接清除MDA外,还与其结构的亲核性有关.本研究从一个新的角度揭示了儿茶素抑制羰-氨交联反应是其预防和治疗羰基应激相关疾病的潜在作用机制之一.
병이철(Malondialdehyde, MDA)시궤체내보편존재적활성탄기화합물대표성분지일,능구여단백질발생탄-안교련반응,생성노년색소형광물질.본문탐토인다소단체성분대MDA응격인기적단백질류노년색소형광물질생성체계적영향,병대기구효관계진행초보분석.결과표명:인다소능현저억제MDA유도적단백질류노년색소물질생성,차지형인다소활성교강,억제작용제여직접청제MDA외,환여기결구적친핵성유관.본연구종일개신적각도게시료인다소억제탄-안교련반응시기예방화치료탄기응격상관질병적잠재작용궤제지일.
Malondialdehyde (MDA) is one of the active carbonyl compounds existing in human body, which can react with protein to form age pigment fluorescence products (APFs) by carbonyl-ammonia cross linking reaction. The purpose of this study is to determine whether tea catechins can inhibit the formation of the age pigment fluorescence products induced by MDA-modified human serum albumin (HSA). Results showed that tea catechins, especially the gallated-catechin, could inhibit the formation of APFs. The structure-activity relationship studies suggested that the inhibiting effects of tea catechins on the formation of APFs were correlated with their nucleophilicity, besides their trapping MDA directly. This research indicated that inhibiting carbonyl-ammonia cross linking reaction may be one of the mechanisms of tea catechins preventing and curing diseases resulting from carbonyl stress reaction.