中国粮油学报
中國糧油學報
중국량유학보
JOURNAL OF THE CHINESE CEREALS AND OILS ASSOCIATION
2010年
2期
54-56,61
,共4页
固体核磁共振%淀粉%双螺旋结构
固體覈磁共振%澱粉%雙螺鏇結構
고체핵자공진%정분%쌍라선결구
solid-state NMR%potato starch%double-helical conformation
采用固体核磁共振技术(solid-state ~(13)C CP/MAS NMR)分别对天然马铃薯淀粉和无定型马铃薯淀粉进行测试并对得到的图谱进行分峰和拟合,以无定型马铃薯淀粉~(13)C CP/MAS NMR图谱中C4在化学位移为84处的值为参考点,用Excel差减法从天然马铃薯淀粉图谱中减去无定型部分,再从得到的结晶淀粉图谱中分出由单螺旋和双螺旋结构所引起的峰,从而通过峰面积的比值计算出天然马铃薯淀粉中所含双螺旋结构的比例.
採用固體覈磁共振技術(solid-state ~(13)C CP/MAS NMR)分彆對天然馬鈴藷澱粉和無定型馬鈴藷澱粉進行測試併對得到的圖譜進行分峰和擬閤,以無定型馬鈴藷澱粉~(13)C CP/MAS NMR圖譜中C4在化學位移為84處的值為參攷點,用Excel差減法從天然馬鈴藷澱粉圖譜中減去無定型部分,再從得到的結晶澱粉圖譜中分齣由單螺鏇和雙螺鏇結構所引起的峰,從而通過峰麵積的比值計算齣天然馬鈴藷澱粉中所含雙螺鏇結構的比例.
채용고체핵자공진기술(solid-state ~(13)C CP/MAS NMR)분별대천연마령서정분화무정형마령서정분진행측시병대득도적도보진행분봉화의합,이무정형마령서정분~(13)C CP/MAS NMR도보중C4재화학위이위84처적치위삼고점,용Excel차감법종천연마령서정분도보중감거무정형부분,재종득도적결정정분도보중분출유단라선화쌍라선결구소인기적봉,종이통과봉면적적비치계산출천연마령서정분중소함쌍라선결구적비례.
The solid-state ~(13)C CP/MAS NMR technique was adopted to examine natural potato starch and amorphous potato starch and to conduct peak fitting on the obtained spectra. The starch spectra were separated into amorphous and ordered sub-spectra using the intensity at 84 of amorphous potato starch as a reference point. The ordered sub-spectra showed the presence of both double helices and Ⅴ-type single helices. The relative proportion of double-helical conformation in natural potato starch was estimated by calculating the ratio of peak area.
