CAJ | 학술논문

[目的]克隆并分析二化螟线粒体细胞色素c氧化酶亚基Ⅰ基因(cox1).[方法]利用PCR方法扩增了二化螟线粒体cox1基因,并测定了其全序列.通过检索GenBank数据库获得了其他21种鳞翅目昆虫的cox1序列,并进行了同源性比较和分子系统学分析.[结果]cox1基因编码框包含1 531个核苷酸,编码510个氨基酸的蛋白;起始密码子为CGA,终止密码子仅由一个T组成.利用ML方法构建了基于cox1基因编码氨基酸序列的鳞翅目昆虫的分子系统树,发现分子系统树与从形态学角度的系统分类在大方面上是基本一致的,但也略有差异.[结论]为进一步研究cox1基因的表达和应用奠定了基础.
[목적]극륭병분석이화명선립체세포색소c양화매아기Ⅰ기인(cox1).[방법]이용PCR방법확증료이화명선립체cox1기인,병측정료기전서렬.통과검색GenBank수거고획득료기타21충린시목곤충적cox1서렬,병진행료동원성비교화분자계통학분석.[결과]cox1기인편마광포함1 531개핵감산,편마510개안기산적단백;기시밀마자위CGA,종지밀마자부유일개T조성.이용ML방법구건료기우cox1기인편마안기산서렬적린시목곤충적분자계통수,발현분자계통수여종형태학각도적계통분류재대방면상시기본일치적,단야략유차이.[결론]위진일보연구cox1기인적표체화응용전정료기출.
[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase Ⅰ gene ( cox 1 ) of C. suppressalis.[Method] The mitochondrial cox 1 gene of C. suppressalis was cloned with PCR method and sequenced. Then, cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C. suppressalis. Finally, homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted. [Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids. The cox1 gene used a start codon CGA, and an incomplete termination codon composed of only T. Based on the amino acid sequences of cox 1, the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood (ML) method. The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly, but also showed some differences. [Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.