CAJ | 학술논문

2006年诱导多能干细胞(iPSCs)的建立被誉为是干细胞研究领域的重大突破,使得进行患者体细胞来源的干细胞治疗成为可能.近年的研究表明,动物和人的成纤维细胞、B淋巴细胞、神经干细胞、头发角质细胞、胰腺细胞、脐带基质和羊膜的间充质细胞均可重编程成为iPSCs并可通过一定的诱导分化为特定类型的细胞,为多种疾病的特异性治疗提供了新的方法.而iPSCs研究在眼科领域的研究也取得了长足进展.在眼科,iPSCs诱导后可分化为视网膜色素上皮(RPE)细胞、感光细胞和其他视网膜细胞,从而为治疗视网膜退行性病变奠定了基础.与传统的干细胞治疗不同,iPSCs移植治疗相应的疾病避免了传统干细胞治疗所存在的道德伦理及免疫排斥等限制,具有较好的应用前景.就iPSCs的概念、诱导策略及方法、在眼科领域的研究现状、研究中存在的问题及应用前景等方面进行综述.
2006년유도다능간세포(iPSCs)적건립피예위시간세포연구영역적중대돌파,사득진행환자체세포래원적간세포치료성위가능.근년적연구표명,동물화인적성섬유세포、B림파세포、신경간세포、두발각질세포、이선세포、제대기질화양막적간충질세포균가중편정성위iPSCs병가통과일정적유도분화위특정류형적세포,위다충질병적특이성치료제공료신적방법.이iPSCs연구재안과영역적연구야취득료장족진전.재안과,iPSCs유도후가분화위시망막색소상피(RPE)세포、감광세포화기타시망막세포,종이위치료시 망막퇴행성병변전정료기출.여전통적간세포치료불동,iPSCs이식치료상응적질병피면료전통간세포치료 소존재적도덕윤리급면역배척등한제,구유교호적응용전경.취iPSCs적개념、유도책략급방법、재안과영역적연구현상、연구중존재적문제급응용전경등방면진행종술.
The establishment of induced pluripotent stem cells(iPSCs)has been a major breakthrough in the field of stem cell research since 2006,and it made possible for the use of stem cells in treating retinal degenerative diseases.Research showed that fibroblast,B lymphocytes,neural stem cells,hair corneous cells,pancreatic cells,mesenchymal cells of umbilical cord stroma and amniotic membrane can be reprogrammed as iPSCs,and they are capable of differentiating into specific types of cells.Some novel developments in iPSCs study in ophthalmology also were observed over the past few years.Induced iPSCs can differentiate into retinal pigment epithelial cells,photoreceptors and other retinal cells,which lay a foundation for the therapy of retinal degenerative diseases.Differented from traditional treatment of stem cells,the generation of iPSCs makes it possible to utilize somatic cells derived from patients for stem cell therapy without provoking ethical and immunological problems.The generation of iPSCs,the current research about iPSCs in the ophthalmic field,the limitations of iPSCs in the clinic and their future development and application were reviewed.