中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
3期
350-353
,共4页
唐昱英%林严俊%李吉满%万俊峰%黄蔚
唐昱英%林嚴俊%李吉滿%萬俊峰%黃蔚
당욱영%림엄준%리길만%만준봉%황위
低温,人工%脑脊髓液%再灌注损伤%脊髓%水通道蛋白质4
低溫,人工%腦脊髓液%再灌註損傷%脊髓%水通道蛋白質4
저온,인공%뇌척수액%재관주손상%척수%수통도단백질4
Hypothermia,induced%Cerebrospinal fluid%Reperfusion injury%Spinal cord%Aqua-porin 4
目的 探讨低温人工脑脊液灌流对兔脊髓缺血再灌注时水通道蛋白-4(AQP-4)表达的影响.方法 成年雄性新西兰大白兔54只,采用随机数字表法,将其随机分为3组(n=18):假手术组(S组)、脊髓缺血再灌注组(I/R组)和低温人工脑脊液灌流组(FI 组).I/R组和FI组采用阻断肾下腹主动脉60 min后开放的方法 制备脊髓缺血再灌注模型.FI组阻断腹主动脉时以30 ml/h的速率经L4,5间隙蛛网膜下腔输注25℃人工脑脊液,同时开放L7,8间隙蛛网膜下腔引流脑脊液.腹主动脉开放的同时停止人工脑脊液灌流.每组取6只动物,分别在再灌注4、24、48、72 h时进行神经功能评分;每组分别于再灌注4、24、72 h时取6只动物,取L5～8脊髓节段,计算脊髓组织含水量,并测定AQP-4表达水平.结果 与S组比较,I/R组神经功能评分降低,脊髓组织含水量增加,AQP-4表达下调(P＜0.05).与I/R组比较,FI组神经功能评分升高,脊髓组织含水量降低,AQP-4表达上调(P＜0.05).结论 低温人工脑脊液灌流可上调AQP-4表达,从而减轻兔脊髓缺血再灌注损伤.
目的 探討低溫人工腦脊液灌流對兔脊髓缺血再灌註時水通道蛋白-4(AQP-4)錶達的影響.方法 成年雄性新西蘭大白兔54隻,採用隨機數字錶法,將其隨機分為3組(n=18):假手術組(S組)、脊髓缺血再灌註組(I/R組)和低溫人工腦脊液灌流組(FI 組).I/R組和FI組採用阻斷腎下腹主動脈60 min後開放的方法 製備脊髓缺血再灌註模型.FI組阻斷腹主動脈時以30 ml/h的速率經L4,5間隙蛛網膜下腔輸註25℃人工腦脊液,同時開放L7,8間隙蛛網膜下腔引流腦脊液.腹主動脈開放的同時停止人工腦脊液灌流.每組取6隻動物,分彆在再灌註4、24、48、72 h時進行神經功能評分;每組分彆于再灌註4、24、72 h時取6隻動物,取L5～8脊髓節段,計算脊髓組織含水量,併測定AQP-4錶達水平.結果 與S組比較,I/R組神經功能評分降低,脊髓組織含水量增加,AQP-4錶達下調(P＜0.05).與I/R組比較,FI組神經功能評分升高,脊髓組織含水量降低,AQP-4錶達上調(P＜0.05).結論 低溫人工腦脊液灌流可上調AQP-4錶達,從而減輕兔脊髓缺血再灌註損傷.
목적 탐토저온인공뇌척액관류대토척수결혈재관주시수통도단백-4(AQP-4)표체적영향.방법 성년웅성신서란대백토54지,채용수궤수자표법,장기수궤분위3조(n=18):가수술조(S조)、척수결혈재관주조(I/R조)화저온인공뇌척액관류조(FI 조).I/R조화FI조채용조단신하복주동맥60 min후개방적방법 제비척수결혈재관주모형.FI조조단복주동맥시이30 ml/h적속솔경L4,5간극주망막하강수주25℃인공뇌척액,동시개방L7,8간극주망막하강인류뇌척액.복주동맥개방적동시정지인공뇌척액관류.매조취6지동물,분별재재관주4、24、48、72 h시진행신경공능평분;매조분별우재관주4、24、72 h시취6지동물,취L5～8척수절단,계산척수조직함수량,병측정AQP-4표체수평.결과 여S조비교,I/R조신경공능평분강저,척수조직함수량증가,AQP-4표체하조(P＜0.05).여I/R조비교,FI조신경공능평분승고,척수조직함수량강저,AQP-4표체상조(P＜0.05).결론 저온인공뇌척액관류가상조AQP-4표체,종이감경토척수결혈재관주손상.
Objective To investigate the effect of irrigation with hypothermic artificial cerebral-spinal fluid (aCSF)on expression of aquaporin-4(AQP-4) in the spinal cord following spinal ischemia-reperfusion (I/R) in rabbits.Methods Fifty-four adult male New Zealand white rabbits weighing 2.0-2.5 kg were randomly divided into 3 groups (n=18 each):group sham operation(S group); group I/R and group irrigation with hypothermic aCSF (FI group). Spinal I/R was induced by clamping the abdominal aorta below renal artery for 60 min. Hypothermic aCSF(25 X.)was infused at L4,5 interspace at a rate of 30 ml/h and drained from L7,8 interspace during spinal ischemia.Neurological function was evaluated at 4, 24, 48 and 72 h of reperfusion and scored (0=no hind limb activity, 4=hind limb function completely recovered) in 6 animals in each group. Six animals were sacrificed at 4, 24 and 72 h respectively in each group.The lumbar segment (L5-8) was removed for measurement of water content and AQP-4 protein expression (by immuno-histochemistry).Results Neurological function scores were significantly lower,water content was higher and AQP-4 expression smaller in group I/R than in group S. I/R-induced effects were significantly attenuated by irrigation of hypothermic aCSF. Conclusion Irrigation with hypothermic aCSF can ameliorate the spinal cord I/R injuries by up-regulation of AQP-4 expression.
