CAJ | 학술논문

目的探讨尖锐湿疣皮损中Toll样受体(TLR)各亚型的表达.方法将尖锐湿疣患者的病损组织标本冰冻切片经免疫组化染色检测10种TLR亚型的表达情况,以正常皮肤组织作对照,比较两者TLR表达谱的差异.结果正常表皮角质层和颗粒层TLR6、9呈强阳性表达,TLR1、3、10呈中等强度表达,TLR2、4、5、7、8弱表达,棘层和基底层细胞不表达或弱表达TLR;尖锐湿疣皮损表皮中除基底层弱表达或不表达TLR外,其余细胞层TLR1、3、6、9强阳性表达,TLR4、8、10中等强度表达,TLR2、5、7弱表达.两组皮肤的真皮层均未见明显TLR阳性细胞.结论尖锐湿疣皮损增生的角质形成细胞中TLR表达较正常皮肤明显增高,尤以TLR1、3、6、9亚型为著,可能与HPV感染启动机体抗病毒免疫有关.
목적 탐토첨예습우피손중Toll양수체(TLR)각아형적표체.방법 장첨예습우환자적병손조직표본빙동절편경면역조화염색검측10충TLR아형적표체정황,이정상피부조직작대조,비교량자TLR표체보적차이.결과 정상표피각질층화과립층TLR6、9정강양성표체,TLR1、3、10정중등강도표체,TLR2、4、5、7、8약표체,극층화기저층세포불표체혹약표체TLR;첨예습우피손표피중제기저층약표체혹불표체TLR외,기여세포층TLR1、3、6、9강양성표체,TLR4、8、10중등강도표체,TLR2、5、7약표체.량조피부적진피층균미견명현TLR양성세포.결론 첨예습우피손증생적각질형성세포중TLR표체교정상피부명현증고,우이TLR1、3、6、9아형위저,가능여HPV감염계동궤체항병독면역유관.
Objective To measure the expressions of Toll like receptors (TLRs) in lesions of condyloma acuminatum (CA). Methods Tissue samples were resected from lesions of 25 patients with CA and normal skin of 16 human controls. Frozen sections were prepared and the expressions of TLRs 1 - 10 were measured by immunohistochemical staining. Results In normal skin samples, there was a strong expression of TLR 6 and 9, medium expression of TLR 1, 3, 10 and weak expression of TLR 2, 4, 5, 7, 8 in the stratum corneum and stratum granulosum, but a weak or negative expression of TLRs in the stratum spinosum and stratum basale. In contrast, a strong expression of TLR 1, 3, 6, 9, medium expression of TLR 4, 8, 10 and weak expression of TLR 2, 5, 7 were detected in keratinocytes of almost all epidermal layers except for stratum basale in CA lesions. Conclusions The expressions of TLRs, especially TLR 1, 3, 6, 9, are markedly enhanced in proliferated keratinocytes in CA lesions compared with normal skin, which is likely to be associated with anti-viral immunity triggered by HPV infection.