CAJ | 학술논문

目的探讨体外培育牛黄复方制剂对血吸虫病门静脉高压肺组织病变干预作用及其机制.方法采用光镜和电镜方法,观察20例血吸虫病门静脉高压家兔毗喹酮对照组和20例体外培育牛黄复方制剂干预组肺组织的形态学改变;用免疫组织化学技术检测20例血吸虫病门静脉高压家兔毗喹酮对照组和20例体外培育牛黄复方制剂干预组肺绀织的纤维连接蛋向(FN)、层粘连蛋白(LN)的表达.结果血吸虫病门静脉高压家兔毗喹酮对照组肺组织呈肺泡腔渗出液较多,可见较多巨噬细胞,肺泡毛细血管扩张,毛细血管三层屏障结构模糊,体外培育牛黄复方制剂干预组肺组织部分区域肺泡间隙纤维轻微增多,I型上皮、基底膜、内皮结构基本完整,肺泡腔无明显渗出;血吸虫病门静脉高压家兔毗喹酮对照组肺组织FN、LN表达呈阳性或强阳性,体外培育牛黄复方制剂干预组肺组织FN、LN则呈阴性或弱阳性表达,两者差异有统计学意义(P<0.01).结论体外培育牛黄复方制剂能够有效预防血吸虫病家兔门脉高压症的肺部并发症,它是通过改善肺微循环、降低细胞外基质含量来抑制肺组织病变.
목적 탐토체외배육우황복방제제대혈흡충병문정맥고압폐조직병변간예작용급기궤제.방법 채용광경화전경방법,관찰20례혈흡충병문정맥고압가토비규동대조조화20례체외배육우황복방제제간예조폐조직적형태학개변;용면역조직화학기술검측20례혈흡충병문정맥고압가토비규동대조조화20례체외배육우황복방제제간예조폐감직적섬유련접단향(FN)、층점련단백(LN)적표체.결과 혈흡충병문정맥고압가토비규동대조조폐조직정폐포강삼출액교다,가견교다거서세포,폐포모세혈관확장,모세혈관삼층병장결구모호,체외배육우황복방제제간예조폐조직부분구역폐포간극섬유경미증다,I형상피、기저막、내피결구기본완정,폐포강무명현삼출;혈흡충병문정맥고압가토비규동대조조폐조직FN、LN표체정양성혹강양성,체외배육우황복방제제간예조폐조직FN、LN칙정음성혹약양성표체,량자차이유통계학의의(P<0.01).결론 체외배육우황복방제제능구유효예방혈흡충병가토문맥고압증적폐부병발증,타시통과개선폐미순배、강저세포외기질함량래억제폐조직병변.
Objective To explore the intervention effects and the mechanism of in vitro cultivated calculus bovis compound preparation (ICCBco) on pulmonary lesion in portal hypertensive rabbits with schistosomiasis. Methods The experimental group included 20 portal hypertensive rabbits with schistosomiasis treated by ICCBco. The control group included 20 portal hypertensive rabbits with schistosomiasis treated by praziquantel.Light and electron microscopy were applied to observe the morphological changes of the pulmonary tissues. The expression of Fibronectin (FN) and Laminin (LN) in the lung tissues was detected by immunohistochemistry. Results More alveolar exudation was found in the lung tissue of the praziquantel control group, and more macrophages, alveolar angiotelectasis and the blurred three-tier structure of alveolar-capillary barrier could also be seen. In ICCBco intervention group fibers within the alveolar interspace had a slight increase in some lung regions. The basic integrity structure of type I epithelium, basement membrane and endodermis were basically intact, and there was no obvious exudation from the alveolar space. There was a positive or strong positive expression of FN and LN in the lung tissue of the praziquantel control group, while a negative or weak positive expression of FN and LN in ICCBco intervention group with the difference being significant (P<0.01). Conclusion ICCBco can effectively prevent pulmonary complications in portal hypertensive rabbits with schistosomiasis by improving lung microcirculation and lowering the content of extracellular matrix.