CAJ | 학술논문

目的探讨DNA甲基化对微RNA(miRNA)表达的影响.方法取2009年7至11月天津医科大学总医院新鲜活检标本胃癌组、对照组各40例.应用甲基化特异性PCR(MSP)方法检测组织中miRNA-34b/c和miRNA-124a基因启动子区甲基化.结果 miRNA-34b/c基因启动子区高甲基化阳性率在胃癌组及对照组胃黏膜中分别为77.5%(31/40)和5.0%(2/40),差异有统计学意义(P<0.05).miRNA-124a基因启动子区高甲基化阳性率在两组中分别为60.0%(24/40)和0,差异有统计学意义(P<0.05).miRNA基因启动子区高甲基化阳性率与胃癌临床病理特征有关.结论胃癌组织存在miRNA-34b/c和miRNA-124a基因启动子区高甲基化,并可能在胃癌的发生发展中起重要作用.
목적 탐토DNA갑기화대미RNA(miRNA)표체적영향.방법 취2009년7지11월천진의과대학총의원신선활검표본위암조、대조조각40례.응용갑기화특이성PCR(MSP)방법검측조직중miRNA-34b/c화miRNA-124a기인계동자구갑기화.결과 miRNA-34b/c기인계동자구고갑기화양성솔재위암조급대조조위점막중분별위77.5%(31/40)화5.0%(2/40),차이유통계학의의(P<0.05).miRNA-124a기인계동자구고갑기화양성솔재량조중분별위60.0%(24/40)화0,차이유통계학의의(P<0.05).miRNA기인계동자구고갑기화양성솔여위암림상병리특정유관.결론 위암조직존재miRNA-34b/c화miRNA-124a기인계동자구고갑기화,병가능재위암적발생발전중기중요작용.
Objective To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma.Methods A total of 80 subjects were divided into gastric carcinoma group(n=40)and control group(n=40).The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues.Results The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5%(31/40)and 5.0%(2/40)in gastric carcinoma and control groups respectively.There was statistically significant difference between two groups (P<0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0%(24/40)and 0 in these two groups respectively.There was statistically significant difference between two groups (P<0.05).Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.Conclusion The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.