中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2009年
3期
197-200
,共4页
水孔蛋白质类%基因表达%羊水过多%胎膜
水孔蛋白質類%基因錶達%羊水過多%胎膜
수공단백질류%기인표체%양수과다%태막
Aquaporins%Gene expression%Polyhydramnios%Extraembryonic membranes
目的 探讨水通道蛋白-1、3、8、9在羊水平衡分子机制中的可能作用.方法 收集足月选择性剖宫产分娩的羊水过多和正常孕妇的胎膜组织样本各5例,运用RT-PCR方法检测胎膜组织中水通道蛋白-1、3、8、9 mRNA的表达.结果 水通道蛋白-9 mRNA的表达水平在羊水过多组胎膜组织的水平(1.1403±0.0831)显著高于对照组(0.5903±0.1909)(P=0.002);水通道蛋白-1、3和8 mRNA在羊水过多组和对照组胎膜的表达差异无统计学意义(P=0.972,0.242,0.608).结论 水通道蛋白-9 mRNA在人羊水过多病例的胎膜组织表达显著升高,在维持羊水量及羊水成分的平衡中可能发挥重要作用.
目的 探討水通道蛋白-1、3、8、9在羊水平衡分子機製中的可能作用.方法 收集足月選擇性剖宮產分娩的羊水過多和正常孕婦的胎膜組織樣本各5例,運用RT-PCR方法檢測胎膜組織中水通道蛋白-1、3、8、9 mRNA的錶達.結果 水通道蛋白-9 mRNA的錶達水平在羊水過多組胎膜組織的水平(1.1403±0.0831)顯著高于對照組(0.5903±0.1909)(P=0.002);水通道蛋白-1、3和8 mRNA在羊水過多組和對照組胎膜的錶達差異無統計學意義(P=0.972,0.242,0.608).結論 水通道蛋白-9 mRNA在人羊水過多病例的胎膜組織錶達顯著升高,在維持羊水量及羊水成分的平衡中可能髮揮重要作用.
목적 탐토수통도단백-1、3、8、9재양수평형분자궤제중적가능작용.방법 수집족월선택성부궁산분면적양수과다화정상잉부적태막조직양본각5례,운용RT-PCR방법검측태막조직중수통도단백-1、3、8、9 mRNA적표체.결과 수통도단백-9 mRNA적표체수평재양수과다조태막조직적수평(1.1403±0.0831)현저고우대조조(0.5903±0.1909)(P=0.002);수통도단백-1、3화8 mRNA재양수과다조화대조조태막적표체차이무통계학의의(P=0.972,0.242,0.608).결론 수통도단백-9 mRNA재인양수과다병례적태막조직표체현저승고,재유지양수량급양수성분적평형중가능발휘중요작용.
Objective To determine the expression of aquaporin-1,3,8,9 mRNA (AQP-1,3,8, 9) in amniotic membranes in pregnant women with polyhydramnios. Methods Amniotic membranes were collected from women who presented with either polyhydramnios (n= 5)or normal amniotic fluid volume (control, n= 5) underwent elective cesarean sections at term. The AQP-1,3,8,9 mRNA expression were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results The expression of AQP-9 mRNA on fetal membranes were significantly higher in polyhydramnios groups (1. 1403±0. 0831) than that of control (0. 5903±0. 1909) (P = 0. 002), although the expression of AQP-1, 3 and 8 showed no significant difference between the two groups (P= 0. 972, 0. 242,0. 608, respectively). Conclusions AQP-9 may play an important role in maintaining the volume and the balance of different components of amniotic fluid in polyhydramnios cases.
