CAJ | 학술논문

目的研究降钙素基因相关肽(CGRP)转基因对同种异体移植血管病变(AV)的防治作用,探讨其作用机制.方法大鼠分3组:(1)转染Ad5-CGRP-EGFP组(A组),(2)转染Ad5-EGFP组(B组),(3)未转染基因组(C组).分别于术后4周、8周取标本,行病理、细胞凋亡检查,观察CGRP、EGFP基因及VCAM-1、iNOS表达.结果 A组术后4周CGRP表达阳性,A组、B组术后4周EGFP基因表达阳性.A组术后4周、8周管腔闭塞指数低于B组和C组.A组术后4周细胞凋亡指数(AI)低于B组和C组.免疫组化显示,A组术后4周VCAM-1表达低于B组和C组,A组iNOS表达仅术后4周内膜低于B组和C组.结论 CGRP基因转染后有效地防治了术后4周AV.
목적 연구강개소기인상관태(CGRP)전기인대동충이체이식혈관병변(AV)적방치작용,탐토기작용궤제.방법 대서분3조:(1)전염Ad5-CGRP-EGFP조(A조),(2)전염Ad5-EGFP조(B조),(3)미전염기인조(C조).분별우술후4주、8주취표본,행병리、세포조망검사,관찰CGRP、EGFP기인급VCAM-1、iNOS표체.결과 A조술후4주CGRP표체양성,A조、B조술후4주EGFP기인표체양성.A조술후4주、8주관강폐새지수저우B조화C조.A조술후4주세포조망지수(AI)저우B조화C조.면역조화현시,A조술후4주VCAM-1표체저우B조화C조,A조iNOS표체부술후4주내막저우B조화C조.결론 CGRP기인전염후유효지방치료술후4주AV.
Objective To explore suppression of allograft vaseulopathy (AV) by transfer of the calcitonin gene-related peptide (CGRP). Methods The descending thoracic aortas from 39 Lewis rats were grafted to the abdominal aortas of 39 F344 rats,and then the recipient rats were randomized into 3 groups:Group A transfected with Ad5-CGRP-EGFP,a gene construct containing sequences from the adenoviral oncoprotein,CGRP,and enhanced green fluorescent protein ,Group B,transfected with Ad5-EGFP containing sequences from the adenoviral oncoprotein and enhanced green fluorescent protein,and Group C without transfection. Four and 8 weeks later the abdominal aortas of 6 recipient rats from each were collected respectively. The degree of vascular obstruction was observed by microscopy. Frozen tissue sections were made and the inverted phase contrast fluorescence microscopy was used to observe the green fluorescence showing the expression of CGRP and enhanced green fluorescent protein (EGFP). RT-PCR was used to detect the expression of CGRP. Immunohistochemistry was used to examine the expression of inducible nitric oxide synthase (iNOS) and vascular cell adhesion molecule-1 (VCAM-1). The apoptosis index (AI) was detected by TUNEL method. Results CGRP expression was positive in both Group A and B 4 weeks later and negative in both groups 8 weeks later. Group A is approximately normal in pathological morphology 4 weeks later. The vascular luminal occlusion score of Group A was lower than Groups B and C 4 weeks later,and significantly higher than that Ⅰ the same group (P<0.05),however,still lower than those of Groups B and C 8 weeks later. The AI 4 weeks later of Group A was significantly lower than those of Groups B and group C,however,there was no significance in AI among the 3 groups. Four weeks later the VCAM-1 expression levels in the tunicae intima,media,and adventita of Group A were all significantly lower than those of Group B C 4 weeks later,however,the iNOS expression level of Group A was only significantly lower than those of Groups B and C in the tunica intima 4 weeks later. Conclusion The expression of CGRP effectively suppresses the development of AV 4 weeks after the operation.