中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
12期
902-905
,共4页
白震民%唐强%李曦%陈世龙
白震民%唐彊%李晞%陳世龍
백진민%당강%리희%진세룡
电针%胰岛素抵抗%胰岛素信号转导途径%葡萄糖转运蛋白4%蛋白激酶Bβ
電針%胰島素牴抗%胰島素信號轉導途徑%葡萄糖轉運蛋白4%蛋白激酶Bβ
전침%이도소저항%이도소신호전도도경%포도당전운단백4%단백격매Bβ
Electroacupuncture%Insulin resistance%Insulin signal transduction pathway%Glucose transporter 4%Protein kinase Bβ
目的 观察电针对胰岛素抵抗(IR)大鼠胰岛素信号转导途径中葡萄糖转运蛋白4(GLUT4)及蛋白激酶Bβ(Akt2)表达的影响.方法 共选取24只健康Wistar雄性大鼠,采用随机数字表法将其分为正常对照组、模型组及电针组,每组8只.采用高脂膳食喂养将模型组及电针组大鼠制成IR模型,电针组于制模成功后给予电针背俞穴治疗.于电针治疗2周后检测各组大鼠胰岛素敏感性指数(ISI);选用逆转录聚合酶链反应(RT-PCR)对各组大鼠骨骼肌细胞GLUT4及Akt2 mRNA表达进行定量分析.结果 模型组大鼠血浆胰岛素(FINS)较正常对照组明显升高(P＜0.05),ISI较正常对照组显著降低(P＜0.05);电针组大鼠经电针治疗2周后,发现其FINS较模型组明显降低(P＜0.05),ISI则显著升高(P＜0.05);并且电针组骨骼肌细胞中GLUT4及Akt2 mRNA表达均显著强于模型组水平(P＜0.05).结论 电针治疗能改善IR模型大鼠病情,其治疗机制可能与电针促进胰岛素磷脂酰肌醇-3激酶途径通路中GLUT4转位有关.
目的 觀察電針對胰島素牴抗(IR)大鼠胰島素信號轉導途徑中葡萄糖轉運蛋白4(GLUT4)及蛋白激酶Bβ(Akt2)錶達的影響.方法 共選取24隻健康Wistar雄性大鼠,採用隨機數字錶法將其分為正常對照組、模型組及電針組,每組8隻.採用高脂膳食餵養將模型組及電針組大鼠製成IR模型,電針組于製模成功後給予電針揹俞穴治療.于電針治療2週後檢測各組大鼠胰島素敏感性指數(ISI);選用逆轉錄聚閤酶鏈反應(RT-PCR)對各組大鼠骨骼肌細胞GLUT4及Akt2 mRNA錶達進行定量分析.結果 模型組大鼠血漿胰島素(FINS)較正常對照組明顯升高(P＜0.05),ISI較正常對照組顯著降低(P＜0.05);電針組大鼠經電針治療2週後,髮現其FINS較模型組明顯降低(P＜0.05),ISI則顯著升高(P＜0.05);併且電針組骨骼肌細胞中GLUT4及Akt2 mRNA錶達均顯著彊于模型組水平(P＜0.05).結論 電針治療能改善IR模型大鼠病情,其治療機製可能與電針促進胰島素燐脂酰肌醇-3激酶途徑通路中GLUT4轉位有關.
목적 관찰전침대이도소저항(IR)대서이도소신호전도도경중포도당전운단백4(GLUT4)급단백격매Bβ(Akt2)표체적영향.방법 공선취24지건강Wistar웅성대서,채용수궤수자표법장기분위정상대조조、모형조급전침조,매조8지.채용고지선식위양장모형조급전침조대서제성IR모형,전침조우제모성공후급여전침배유혈치료.우전침치료2주후검측각조대서이도소민감성지수(ISI);선용역전록취합매련반응(RT-PCR)대각조대서골격기세포GLUT4급Akt2 mRNA표체진행정량분석.결과 모형조대서혈장이도소(FINS)교정상대조조명현승고(P＜0.05),ISI교정상대조조현저강저(P＜0.05);전침조대서경전침치료2주후,발현기FINS교모형조명현강저(P＜0.05),ISI칙현저승고(P＜0.05);병차전침조골격기세포중GLUT4급Akt2 mRNA표체균현저강우모형조수평(P＜0.05).결론 전침치료능개선IR모형대서병정,기치료궤제가능여전침촉진이도소린지선기순-3격매도경통로중GLUT4전위유관.
Objective To explore the effects of electroacupuncture on the expression of glucose transporter 4 (GLUT4) and protein kinase Bβ (Akt2) in the skeletal muscles of insulin-resistant (IR) rats. Methods Twentyfour male Wistar rats were randomly divided into a normal control group, a model group and an electroacupuncture (EA) group. The rats of the model group and the EA group were fed with high fat diets to establish a model of insulin resistance. The rats in the EA group were then treated with electroacupuncture for 2 weeks, while those in the model group were not. Blood samples were collected to evaluate fasting insulin (FINS) and fasting blood glucose (FBG) to calculate the insulin sensitivity index (ISI). GLUT4 and Akt2 mRNA in the skeletal muscles were determined with reverse transcription-polymerase chain reactions (RT-PCRs) after 2 weeks of EA treatment. Results Compared with the control group, the FINS in the model group increased significantly, and ISI decreased significantly. Compared with the model group, the FINS in the EA group decreased significantly and ISI increased significantly. The expression of GLUT4 and Akt2 mRNA in the model group was significantly lower than in the control group or the EA group. Conclusion Electroacupuncture might improve the condition of IR rats, probably by enhancing the transposition of GLUT4 in the phosphatidylinositol 3-kinase/protein kinase signaling pathway.
