感染后肠易激综合征小鼠模型建立过程中肠道细胞因子表达的变化
감염후장역격종합정소서모형건립과정중장도세포인자표체적변화
Altered expression of intestinal cytokines in development of postinfectious irritable bowel syndrome mouse model
  • 万方数据
    中华医学杂志 중화의학잡지
    National Medical Journal of China
    2010年 34期 2415-2419 ,共5页

    龙艳芹%童晶晶%钱伟%侯晓华

    룡염근%동정정%전위%후효화

    毛绒虫,旋%感染%肠易激综合征%细胞因子类%内脏敏感性 毛絨蟲,鏇%感染%腸易激綜閤徵%細胞因子類%內髒敏感性
    모융충,선%감염%장역격종합정%세포인자류%내장민감성
    Trichinella spiralis%Infection%Irritable bowel syndrome%Cytokines%Visceral sensitivity
    目的 观察旋毛虫急性感染美国国立卫生院(NIH)小鼠建立感染后肠易激综合征模型的不同阶段,肠道组织中Th1类细胞因子白细胞介素(IL)-12和Th2类细胞因子IL-4表达的变化.方法 旋毛虫幼虫囊胞(350～400条)感染成年NIH小鼠,分别于感染后0、2、4、8、12周测量小鼠体重,并于每个观察时间点测最腹壁回撤反应(AWR)以评估感染后小鼠对结直肠扩张的内脏敏感性,于不同时间点处死小鼠取末端回肠行病理切片HE染色观察肠道炎症情况.应用反转录-聚合酶链反应(RT-PCR)和Western印迹检测不同时间点小鼠末端回肠IL-12、IL-4的mRNA和蛋白的表达量.结果 感染后2、4周小鼠体重均低于对照组[(31.1±3.7)g比(35.6±2.7)g,(36.1±3.4)g比(39.8±2.7)g,均P<0.05],感染后8、12周小鼠体重与对照组差异均无统计学意义(均P>0.05).感染后2周肠道急性炎症明显,4周炎症开始恢复,感染后8周至12周肠道无明显炎症表现.在结直肠扩张压力为30、45、60 mm Hg(1 mm Hg=0.133 kPa)时,感染后各时间点小鼠AWR评分均高于对照组,其中以2周时为最高(2.60±0.55比1.00±0.35,2.90±0.20比1.50±0.70,3.30±0.50比2.00±0.35,均P<0.05).感染后8周可作为感染后肠易激综合征的成功模型.感染后小鼠末端回肠组织中IL-12 mRNA及蛋白表达量均高于对照组(均P<0.05),其中以2周时为最高(0.77±0.04,0.40±0.05),4周(0.45±0.04,0.30±0.07)、8周(0.42±0.03,0.25±0.05)、12周(0.39±0.02,0.24±0.04)时依次降低但仍均高于对照(0.34±0.04,0.15±0.07,均P<0.05);IL-4 mRNA及蛋白表达在2周时升高(0.42±0.04,0.33±0.05),4周时(0.20±0.02,0.14±0.02)接近对照组(0.19±0.04,0.13±0.06),8周(0.10±0.03,0.08±0.03)至12周(0.08±0.03,0.06±0.03)时降低(均P<0.05).结论 感染后肠易激综合征模型小鼠肠道组织中Th类细胞因子在模型发展的不同阶段处于不同的表达水平,急性期均表达增强,模型建立成功后Th1表达持续增强而Th2表达持续降低.
    목적 관찰선모충급성감염미국국립위생원(NIH)소서건립감염후장역격종합정모형적불동계단,장도조직중Th1류세포인자백세포개소(IL)-12화Th2류세포인자IL-4표체적변화.방법 선모충유충낭포(350～400조)감염성년NIH소서,분별우감염후0、2、4、8、12주측량소서체중,병우매개관찰시간점측최복벽회철반응(AWR)이평고감염후소서대결직장확장적내장민감성,우불동시간점처사소서취말단회장행병리절편HE염색관찰장도염증정황.응용반전록-취합매련반응(RT-PCR)화Western인적검측불동시간점소서말단회장IL-12、IL-4적mRNA화단백적표체량.결과 감염후2、4주소서체중균저우대조조[(31.1±3.7)g비(35.6±2.7)g,(36.1±3.4)g비(39.8±2.7)g,균P<0.05],감염후8、12주소서체중여대조조차이균무통계학의의(균P>0.05).감염후2주장도급성염증명현,4주염증개시회복,감염후8주지12주장도무명현염증표현.재결직장확장압력위30、45、60 mm Hg(1 mm Hg=0.133 kPa)시,감염후각시간점소서AWR평분균고우대조조,기중이2주시위최고(2.60±0.55비1.00±0.35,2.90±0.20비1.50±0.70,3.30±0.50비2.00±0.35,균P<0.05).감염후8주가작위감염후장역격종합정적성공모형.감염후소서말단회장조직중IL-12 mRNA급단백표체량균고우대조조(균P<0.05),기중이2주시위최고(0.77±0.04,0.40±0.05),4주(0.45±0.04,0.30±0.07)、8주(0.42±0.03,0.25±0.05)、12주(0.39±0.02,0.24±0.04)시의차강저단잉균고우대조(0.34±0.04,0.15±0.07,균P<0.05);IL-4 mRNA급단백표체재2주시승고(0.42±0.04,0.33±0.05),4주시(0.20±0.02,0.14±0.02)접근대조조(0.19±0.04,0.13±0.06),8주(0.10±0.03,0.08±0.03)지12주(0.08±0.03,0.06±0.03)시강저(균P<0.05).결론 감염후장역격종합정모형소서장도조직중Th류세포인자재모형발전적불동계단처우불동적표체수평,급성기균표체증강,모형건립성공후Th1표체지속증강이Th2표체지속강저.
    Objective To observe the expression of Th1 type cytokine IL-12 and Th2 type cytokine IL-4 in different development phases of postinfectious irritable bowel syndrome in mouse model.Methods Mice were infected by Trichinella spiralis (350-400 Trichinella) and weighted weekly after infection.Visceral sensitivity of colorectal distention in mice was assessed by abdominal withdrawal reflex (AWR) at Weeks 0,2,4,8,12 post-infection.Tissues of terminal ileum were collected.Histological pathology and inflammation were evaluated by HE staining.Results The weights of mice in 2 and 4-week groups were lower than those in the control group[(31.1±3.7) g vs (35.6±2.7) g,(36.1±3.4) g vs (39.8±2.7) g,all P < 0.05)].The weights of 8,12-week groups had no statistical difference with the control group (all P > 0.05).Severe intestinal inflammation was observed at Week 2 during acute infectious period,but after a 4-week infection it recovered from intestinal inflammation,until Weeks 8-12,there was no difference with the control group.At 30,45,60 mm Hg,the AWR scores of the infectious group was higher than those in the control group.The 2-week group was the highest (2.60 ±0.55 vs 1.00 ±0.35,2.90 ±0.20 vs 1.50 ±0.70,3.30±0.50 vs 2.00±0.35,all P < 0.05).Mice infected at Week 8 could serve as a successful model of postinfectious irritable bowel syndrome.An elevated expression of IL-12,IL-4 mRNA and protein was observed in ileocecum at Week 2 during acute phase (0.77±0.04 and 0.40±0.05,0.42±0.04 and 0.33 ±0.05),decreased expression of IL-4 mRNA and protein was observed in ileocecum at Weeks 8,12(0.10±0.03 and 0.08 ±0.03,0.08 ±0.03 and 0.06 ±0.03).However a prolonged high expression of IL-12 mRNA and protein was observed in ileocecum at Weeks 8,12 (0.42±0.03 and 0.25±0.05,0.39±0.02 and 0.24±0.04),but lower than those in the 2-week group (all P < 0.05).Conclusion A differential expression of Th type cytokines is observed in different development phases of postinfectious irritable bowel syndrome in a mouse model.All cytokines increase during acute infection stage; However Th1type cytokine increases continuously while Th2 type cytokine decreases in the established model.
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