中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2008年
24期
1923-1926
,共4页
董字启%李宝娥%刘宣勇%冯宇%曹聪
董字啟%李寶娥%劉宣勇%馮宇%曹聰
동자계%리보아%류선용%풍우%조총
庆大霉素类%银%等离子体喷涂%硅灰石涂层%抗菌
慶大黴素類%銀%等離子體噴塗%硅灰石塗層%抗菌
경대매소류%은%등리자체분도%규회석도층%항균
Gentamicins%Silver%Plasma spray%Wollastonite coating%Antibacterial
目的 制备具有长效药物缓释效果的骨科抗菌植入材料表面涂层.方法 采用等离子体喷涂技术在钛合金表面沉积硅灰石涂层.一组试样在浓度为重量百分率5%的硝酸银溶液中浸泡24 h获得载银硅灰石涂层,另一组试样通过与装载庆大霉素的胶原溶液发生接枝反应制得载庆大霉素硅灰石涂层.两种涂层的性能通过抗菌剂体外溶液释放实验、抗会黄色葡萄球菌的抑菌环实验和成骨细胞接触培养毒性实验进行表征.结果 成功制备载银与载庆大霉素硅灰石涂层.载银硅灰石涂层均匀释放银离子达50 d;涂层抗金黄色葡萄球菌能力可维持40 d以上.载庆大霉素硅灰石涂层呈爆发性释放庆大霉素,涂层抗会黄色葡葡球菌能力可以维持18 d.两种涂层均不影响成骨细胞在其表面的黏附、增殖以及碱性磷酸酶活性的表达.结论 载银硅灰石涂层与载庆大霉素硅厌石涂层相比,抗菌剂释放较均匀、抗菌时效较长,更具临床应用前景.
目的 製備具有長效藥物緩釋效果的骨科抗菌植入材料錶麵塗層.方法 採用等離子體噴塗技術在鈦閤金錶麵沉積硅灰石塗層.一組試樣在濃度為重量百分率5%的硝痠銀溶液中浸泡24 h穫得載銀硅灰石塗層,另一組試樣通過與裝載慶大黴素的膠原溶液髮生接枝反應製得載慶大黴素硅灰石塗層.兩種塗層的性能通過抗菌劑體外溶液釋放實驗、抗會黃色葡萄毬菌的抑菌環實驗和成骨細胞接觸培養毒性實驗進行錶徵.結果 成功製備載銀與載慶大黴素硅灰石塗層.載銀硅灰石塗層均勻釋放銀離子達50 d;塗層抗金黃色葡萄毬菌能力可維持40 d以上.載慶大黴素硅灰石塗層呈爆髮性釋放慶大黴素,塗層抗會黃色葡葡毬菌能力可以維持18 d.兩種塗層均不影響成骨細胞在其錶麵的黏附、增殖以及堿性燐痠酶活性的錶達.結論 載銀硅灰石塗層與載慶大黴素硅厭石塗層相比,抗菌劑釋放較均勻、抗菌時效較長,更具臨床應用前景.
목적 제비구유장효약물완석효과적골과항균식입재료표면도층.방법 채용등리자체분도기술재태합금표면침적규회석도층.일조시양재농도위중량백분솔5%적초산은용액중침포24 h획득재은규회석도층,령일조시양통과여장재경대매소적효원용액발생접지반응제득재경대매소규회석도층.량충도층적성능통과항균제체외용액석방실험、항회황색포도구균적억균배실험화성골세포접촉배양독성실험진행표정.결과 성공제비재은여재경대매소규회석도층.재은규회석도층균균석방은리자체50 d;도층항금황색포도구균능력가유지40 d이상.재경대매소규회석도층정폭발성석방경대매소,도층항회황색포포구균능력가이유지18 d.량충도층균불영향성골세포재기표면적점부、증식이급감성린산매활성적표체.결론 재은규회석도층여재경대매소규염석도층상비,항균제석방교균균、항균시효교장,경구림상응용전경.
Objective To develop antibacterial coatings for orthopedic implants with a sustained release of drugs. Methods Wollastonite coatings were deposited on the titanium substrates by an atmospheric plasma spray system. After soaking in weight percent of 5% AgNO3 solution for 24 h, the wollastonite coatings loading silver were obtained.Gentamicin were loaded on the wollastonite coatings by collagen grafting process.The release rates of drugs from wollastonite coatings were investigated by the in vitro solution soaking test. One strain of S. aureus was used in zone of inhibition test to evaluate the antibacterial properties of drug loaded wollastonite coatings, and the cell culture test was used to evaluate their cytotoxicity.Results Silver and gentamicin loaded wollastonite coatings were successfully prepared.The release of silver ions from the silver loaded wollastonite coatings lasted 50 d in deionized water, effectively inhibiting the growth of S.aureus for 40 d.While an initial burst release of gentamicin was found during the in vitro solution soaking test.The gentamicin released from gentamicin loaded wollastonite coatings can inhibit the growth of S.aureus for 18 d.Both the two kinds of antibacterial wollastonite coatings showed no adverse effect on cellular adhesion, proliferation and alkaline phosphatase expression. Conclusions Compared with gentamicin loaded wollastonite coatings, silver loaded wollastonite coatings may have more promising clinical applications due to the even and long-time antibacterial agent release.
