CAJ | 학술논문

目的检测肝细胞癌(hepatocellular carcinoma，HCC)患者术前血清碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)水平，确定其与HCC根治性切除术后转移复发的关系。方法采用Cox比例风险模型，对影响预后的临床指标进行分析，确定影响HCC切除术后转移复发的临床指标。同时应用ELISA方法，检测HCC患者术前血清bFGF水平，western blot方法对24例相应肿瘤组织bFGF表达水平进行定量分析。结果Cox比例风险模型显示，肿瘤大小及肿瘤是否存在肝内播散，是影响HCC切除术后患者生存时间的显著因素(分别为P=0.022和P=0.040)，并以此划分为高和低转移复发倾向组。根治性切除术后高转移复发倾向组，HCC患者术前血清bFGF显著高于低转移复发倾向组(P＜0.01)。western blot显示，HCC患者血清bFGF水平变化与其对应肿瘤组织bFGF表达变化相一致。结论HCC患者术前血清bFGF水平是预测HCC根治性切除术后转移复发的潜在指标。HCC患者血清bFGF水平升高是其对应肿瘤组织高表达的结果。
목적검측간세포암(hepatocellular carcinoma，HCC)환자술전혈청감성성섬유세포생장인자(basic fibroblast growth factor，bFGF)수평，학정기여HCC근치성절제술후전이복발적관계。방법채용Cox비례풍험모형，대영향예후적림상지표진행분석，학정영향HCC절제술후전이복발적림상지표。동시응용ELISA방법，검측HCC환자술전혈청bFGF수평，western blot방법대24례상응종류조직bFGF표체수평진행정량분석。결과Cox비례풍험모형현시，종류대소급종류시부존재간내파산，시영향HCC절제술후환자생존시간적현저인소(분별위P=0.022화P=0.040)，병이차화분위고화저전이복발경향조。근치성절제술후고전이복발경향조，HCC환자술전혈청bFGF현저고우저전이복발경향조(P＜0.01)。western blot현시，HCC환자혈청bFGF수평변화여기대응종류조직bFGF표체변화상일치。결론HCC환자술전혈청bFGF수평시예측HCC근치성절제술후전이복발적잠재지표。HCC환자혈청bFGF수평승고시기대응종류조직고표체적결과。
Objective To study the significance of serum basic fibroblast growth factor (bFGF) as a predictor for metastatic recurrence after curative resection of hepatocellular carcinoma (HCC). Method Cox's proportional hazard model was used to determine the significant clinical factors of prognosis. Meanwhile, enzyme linked immunosorbent assay (ELISA) and Western Blot analysis were used to test serum and tumor tissue levels of bFGF respectively in HCC patients. Results Cox's proportional hazard model showed that tumor size and tumor number were significant prognostic factors of HCC patients after hepatic resection ( P = 0. 022 and P = 0. 040, respectively). Based on this results, HCC patients in our studies were divided into high-tendency to metastatic recurrence and low-tendency to metastatic recurrence groups. Serum levels of bFGF in high-tendency to metastatic recurrence group were significantly higher than those in low-tendency to metastatic recurrence group (P ＜ 0.01 ). Western Blot analysis revealed that changes of bFGF level in the serum were consistent with those in the tumor tissues of HCC patients. Conclusion Serum bFGF is a potential predictor of metastatic recurrence after curative resection of HCC. The higher levels of bFGF in the serum are the results of higher bFGF expressions in the tumor tissues of HCC patients.