中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2009年
12期
1106-1109
,共4页
成纤维细胞%瘢痕%肌动蛋白类%胶原Ⅲ型
成纖維細胞%瘢痕%肌動蛋白類%膠原Ⅲ型
성섬유세포%반흔%기동단백류%효원Ⅲ형
Fibroblasts%Cicatrix%Acting%Collagen type Ⅲ
目的 探讨表达α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的肌成纤维细胞在皮肤增牛性瘢痕中的作用.方法 免疫组织化学法检测皮肤增牛性瘢痕中α-SMA的表达;通过细胞培养,检测成纤维细胞与肌成纤维细胞羟脯氨酸含量并进行比较;ELISA法分别检测成纤维细胞与肌成纤维细胞Ⅲ型前胶原、纤维连接蛋白、TGF-β1含量并进行比较.结果 正常皮肤组织只有血管平滑肌细胞表达α-SMA,其他真皮细胞几乎不表达,增生性瘢痕皮肤组织中,α-SMA染色阳性细胞大大增加,比例高达96.89%,两者比较,差异有统计学意义(P<0.01);肌成纤维细胞羟脯氨酸含量较正常成纤维细胞显著增高(P<0.01);肌成纤维细胞Ⅲ型前胶原含量较成纤维细胞显著增高(P<0.05);肌成纤维细胞TGF-β1含量较正常成纤维细胞显著增高(P<0.01);肌成纤维细胞纤维连接蛋白含量较成纤维细胞显著增高(P<0.05).结论 肌成纤维细胞是造成增生性瘢痕的最关键细胞.
目的 探討錶達α平滑肌肌動蛋白(α-smooth muscle actin,α-SMA)的肌成纖維細胞在皮膚增牛性瘢痕中的作用.方法 免疫組織化學法檢測皮膚增牛性瘢痕中α-SMA的錶達;通過細胞培養,檢測成纖維細胞與肌成纖維細胞羥脯氨痠含量併進行比較;ELISA法分彆檢測成纖維細胞與肌成纖維細胞Ⅲ型前膠原、纖維連接蛋白、TGF-β1含量併進行比較.結果 正常皮膚組織隻有血管平滑肌細胞錶達α-SMA,其他真皮細胞幾乎不錶達,增生性瘢痕皮膚組織中,α-SMA染色暘性細胞大大增加,比例高達96.89%,兩者比較,差異有統計學意義(P<0.01);肌成纖維細胞羥脯氨痠含量較正常成纖維細胞顯著增高(P<0.01);肌成纖維細胞Ⅲ型前膠原含量較成纖維細胞顯著增高(P<0.05);肌成纖維細胞TGF-β1含量較正常成纖維細胞顯著增高(P<0.01);肌成纖維細胞纖維連接蛋白含量較成纖維細胞顯著增高(P<0.05).結論 肌成纖維細胞是造成增生性瘢痕的最關鍵細胞.
목적 탐토표체α평활기기동단백(α-smooth muscle actin,α-SMA)적기성섬유세포재피부증우성반흔중적작용.방법 면역조직화학법검측피부증우성반흔중α-SMA적표체;통과세포배양,검측성섬유세포여기성섬유세포간포안산함량병진행비교;ELISA법분별검측성섬유세포여기성섬유세포Ⅲ형전효원、섬유련접단백、TGF-β1함량병진행비교.결과 정상피부조직지유혈관평활기세포표체α-SMA,기타진피세포궤호불표체,증생성반흔피부조직중,α-SMA염색양성세포대대증가,비례고체96.89%,량자비교,차이유통계학의의(P<0.01);기성섬유세포간포안산함량교정상성섬유세포현저증고(P<0.01);기성섬유세포Ⅲ형전효원함량교성섬유세포현저증고(P<0.05);기성섬유세포TGF-β1함량교정상성섬유세포현저증고(P<0.01);기성섬유세포섬유련접단백함량교성섬유세포현저증고(P<0.05).결론 기성섬유세포시조성증생성반흔적최관건세포.
Objective To explore the role of myofibroblasts expressing α-smooth muscle actin (a-SMA) in hyperplastic scar formation.Methods Expression of α-SMA in normal skin and scar was examined by means of immunohistochemistry.The contents of myofibroblasts were measured and com-pared with that of hydroxyproline through cell culture.The contents of precollagen (pc β) , transforming growth factor β (TGF-β) and fibronectin in fibroblasts and myofibroblasts were assayed by ELISA.Results Vascular smooth muscle cells rather than other hypodermal cells expressed α-SMA in normal skin (P<0.01).The expression ofa-SMA was markedly increased up to 96.89% in hyperplastic scar tissues, with statistical difference compared with normal skin (P < 0.01).The contents of hydroxyproline (P <0.01) , pc (P <0.05) , TGF-β (P <0.01) and fibronectin (P <0.05) in myofibroblasts were significantly higher than those in fibroblasts.Conclusion Myofibroblasts play a key role in formation of hypertrophic scar.
