CAJ | 학술논문

目的研究骨桥蛋白(OPN)在类风湿关节炎(RA)患者外周血中的浓度变化及在滑膜的表达,探讨OPN在RA患者中的发病机制.方法收集91例RA患者以及29名正常对照人群临床资料和血清,用酶联免疫吸附试验(ELIsA)方法检测OPN在RA患者外周血中的浓度,分析其变化和RA临床及实验室指标的关系.收集7例RA患者和1名正常对照的滑膜组织,用免疫组织化学的方法观察OPN的表达情况.结果与正常对照组相比,活动组和非活动组的RA患者外周血中OPN水平均明显升高(p＜0.01),且与RA患者的部分临床指标有相关性:与压痛关节数(r=0.435,P=0.005)、关节的X线分期(r=-0.415,P=0.007)、关节功能(r=0.394,P=0.012)显著相关.OPN在RA滑膜组织大量表达,而在正常对照仅见OPN的少量表达.结论 OPN在RA患者外周血中浓度显著升高,且在滑膜表达明显,OPN可能与RA滑膜增生、骨侵蚀有关.
목적 연구골교단백(OPN)재류풍습관절염(RA)환자외주혈중적농도변화급재활막적표체,탐토OPN재RA환자중적발병궤제.방법 수집91례RA환자이급29명정상대조인군림상자료화혈청,용매련면역흡부시험(ELIsA)방법검측OPN재RA환자외주혈중적농도,분석기변화화RA림상급실험실지표적관계.수집7례RA환자화1명정상대조적활막조직,용면역조직화학적방법관찰OPN적표체정황.결과 여정상대조조상비,활동조화비활동조적RA환자외주혈중OPN수평균명현승고(p＜0.01),차여RA환자적부분림상지표유상관성:여압통관절수(r=0.435,P=0.005)、관절적X선분기(r=-0.415,P=0.007)、관절공능(r=0.394,P=0.012)현저상관.OPN재RA활막조직대량표체,이재정상대조부견OPN적소량표체.결론 OPN재RA환자외주혈중농도현저승고,차재활막표체명현,OPN가능여RA활막증생、골침식유관.
Objective To explore the change of serum osteopontin concentration and the expression of OPN in the synovial membrane of patients with rheumatoid arthritis.Methods We measured the serum osteo-pontin concentration in 91 RA patients and 29 sex-and age-matched normal control subjects using an en-zyme-linked immunoabsorbent assay and collected clinic data at the same time.The expression of OPN in the synovial membrane from 7 RA patients and 1 patient with trauma was examined by immunohistochemisty.Results Serum osteopontin concentrations were significantly higher in both active and inactive RA patients than that in the normal control group:(65±22)ng/ml in active RA,(56±22)ng/ml in inactive RA patients and(26±8)ng/ml(P＜0.005)in normal controls.Increase in OPN concentration correlated positively and sig-nificantly with the number of tender joints,joint x-ray change stages,and joint function(r=O.435,P=0.005.r=0.415,P=0.007,r=O.394,PP=0.012).The expression of OPN was observed in all samples from patients.OPN was expressed in the synovial lining and sublining layer.Conclusion The above results suggest that the pro-duction of osteopontin is associated with joint destruction in RA.