中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
12期
1057-1059
,共3页
南娟%银杰%刘鹏%张占雀%杜艳霞%邢伟%蔡葵%蔡原%张立峰%时利德
南娟%銀傑%劉鵬%張佔雀%杜豔霞%邢偉%蔡葵%蔡原%張立峰%時利德
남연%은걸%류붕%장점작%두염하%형위%채규%채원%장립봉%시리덕
铝暴露%长时程增强%出生前后
鋁暴露%長時程增彊%齣生前後
려폭로%장시정증강%출생전후
Aluminum exposure%Long-term potentiation%Period from prenatal to postnatal
目的 研究出生前后不同浓度慢性铝暴露对年轻大鼠海马长时程增强的影响,进一步探讨铝损害学习与记忆的突触机制.方法 对照组、低浓度组和高浓度组大鼠从孕期始分别自由饮用蒸馏水、Al3+浓度为15mmol·L-1和30mmol·L-1的AlCl3水溶液;采用细胞外微电极记录法测定海马长时程增强.结果 对照组大鼠海马长时程增强发生率为90.91%,低浓度组为58.33%、高浓度组为18.18%,与对照组相比,高浓度组长时程增强发生率差异有显著性(P<0.01).对照组大鼠群体锋电位平均相对幅值达基线值(144.09±18.43)%,低浓度组为(105.83±16.79)%,高浓度组为(91.00±18.98)%,两铝暴露组与对照组比较,群体锋电位平均相对幅值增强率均明显降低(P<0.01),且各时点相比差异有显著性(P<0.01).结论 出生前后慢性铝暴露损害了海马LTP的诱导与维持,导致学习与记忆功能下降.
目的 研究齣生前後不同濃度慢性鋁暴露對年輕大鼠海馬長時程增彊的影響,進一步探討鋁損害學習與記憶的突觸機製.方法 對照組、低濃度組和高濃度組大鼠從孕期始分彆自由飲用蒸餾水、Al3+濃度為15mmol·L-1和30mmol·L-1的AlCl3水溶液;採用細胞外微電極記錄法測定海馬長時程增彊.結果 對照組大鼠海馬長時程增彊髮生率為90.91%,低濃度組為58.33%、高濃度組為18.18%,與對照組相比,高濃度組長時程增彊髮生率差異有顯著性(P<0.01).對照組大鼠群體鋒電位平均相對幅值達基線值(144.09±18.43)%,低濃度組為(105.83±16.79)%,高濃度組為(91.00±18.98)%,兩鋁暴露組與對照組比較,群體鋒電位平均相對幅值增彊率均明顯降低(P<0.01),且各時點相比差異有顯著性(P<0.01).結論 齣生前後慢性鋁暴露損害瞭海馬LTP的誘導與維持,導緻學習與記憶功能下降.
목적 연구출생전후불동농도만성려폭로대년경대서해마장시정증강적영향,진일보탐토려손해학습여기억적돌촉궤제.방법 대조조、저농도조화고농도조대서종잉기시분별자유음용증류수、Al3+농도위15mmol·L-1화30mmol·L-1적AlCl3수용액;채용세포외미전겁기록법측정해마장시정증강.결과 대조조대서해마장시정증강발생솔위90.91%,저농도조위58.33%、고농도조위18.18%,여대조조상비,고농도조장시정증강발생솔차이유현저성(P<0.01).대조조대서군체봉전위평균상대폭치체기선치(144.09±18.43)%,저농도조위(105.83±16.79)%,고농도조위(91.00±18.98)%,량려폭로조여대조조비교,군체봉전위평균상대폭치증강솔균명현강저(P<0.01),차각시점상비차이유현저성(P<0.01).결론 출생전후만성려폭로손해료해마LTP적유도여유지,도치학습여기억공능하강.
Objective To study the change of LTP in hippocampus of young rats after chronic aluminum exposure of different concentration from prenatal to postnatal,and to go deep into the synaptic mechanism of that aluminum impairs learning and memory capacity.Methods The extracellular mieroelectrode technique was used to monitor the LTP.Results The occurrence of LTP in control group was 90.91%,in 0.2%-Al group was 58.33%,in 0.4%-Al group was 18.18%,compared with the control group,in 0.4%-Al group,the occurrence of LTP was remarkably decreased(P<0.01).The PS mean amplitudes relative to the baseline vAlues after HFS in control group was(144.09±18.43)%,in 0.2%.Al group Was (105.83±16.79)%,in 0.4%-Al group Was (91.00±18.98)%.compared with the control group,in the Al3+ exposed groups,the PS mean amplitudes relative to the haseline values were significanfly decreased(P<0.01),and the mean relative amplitude differences in relation to the baseline values at selected time periods after HFS were significantly decreased(P<0.01).Conclusion Aluminum exposure from prenatal to postnatal can damage the induction and maintenace of LTP in hippocampus of young rats.consquentely impair the ability of learning and memory.
