CAJ | 학술논문

目的探讨神经生长因子(NGF)和脑源性神经营养因子(BDNF)在氧化应激诱导细胞凋亡中的作用。方法脂质体介导人NGF和BDNF基因转染鼠成纤维细胞NIH 3T3，使目的基因在细胞中表达，过氧化氢(H2O2)处理转染细胞，吖啶橙荧光染色法和流式细胞仪检测细胞凋亡。结果 50 μmol/L H2O2能诱导NIH 3T3细胞凋亡，处理后24 h凋亡率为16.7%，而NIH 3T3细胞分别经NGF、BDNF和NGF/BDNF转染表达目的基因后，能对抗H 2O2诱导的细胞凋亡，其24 h凋亡率分别为8.21%、9.51%和6.26%。结论氧化应激能诱导细胞凋亡，NGF和BDNF能抑制氧化应激诱导的细胞凋亡，两者具有协同作用。
목적탐토신경생장인자(NGF)화뇌원성신경영양인자(BDNF)재양화응격유도세포조망중적작용。방법지질체개도인NGF화BDNF기인전염서성섬유세포NIH 3T3，사목적기인재세포중표체，과양화경(H2O2)처리전염세포，아정등형광염색법화류식세포의검측세포조망。결과 50 μmol/L H2O2능유도NIH 3T3세포조망，처리후24 h조망솔위16.7%，이NIH 3T3세포분별경NGF、BDNF화NGF/BDNF전염표체목적기인후，능대항H 2O2유도적세포조망，기24 h조망솔분별위8.21%、9.51%화6.26%。결론양화응격능유도세포조망，NGF화BDNF능억제양화응격유도적세포조망，량자구유협동작용。
Objective　To investigate the effects of nerve growth fact or(NGF) and brain-derived neurothophic factor (BDNF) on oxidative stress-induc ed cell apoptosis. 　Methods　 After transfection of hNGF or/and hBDNF cDNA mediat ed by Lipofect AMINE, fibroblast cells were treated with 50 μmol/L H2O2, an d then , apoptosis of the cells was observed by fluorescence staining with acridine ora nge morphologically and flow cytometry analysis. 　Results　 Apo ptosis of fibroblast cells was induced by 50 μmol/L H2O2 and the rate of ap o ptosis 24 h after treatment was 16.7%. Fibroblast cells expression of exogen ous NGF or/and BDNF was prevented from the H2O2-induced apoptosis, the rate s of apoptosis of the 3 groups (NGF transfection, BDNF transfection and NGF & BD NF cotransfection) were 8.21%, 9.51% and 6.26%, respectively 24 h af ter H2O2 treatment. 　Conclusions　 NGF or/and BDNF can protect fibroblast cells fro m oxidative stress-induced cell death, and there is coordination between NGF an d BDNF in protecting against oxidative stress.