农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2012年
1期
79-81
,共3页
王小兰%唐馨%刘忠渊
王小蘭%唐馨%劉忠淵
왕소란%당형%류충연
OsWRKY17基因%GFP基因%表达载体
OsWRKY17基因%GFP基因%錶達載體
OsWRKY17기인%GFP기인%표체재체
OsWRKY17%Green fluorescent protein gene%Expression vector
[目的]研究水稻OsWRKY17基因的生理生化特性,确定OsWRKY17蛋白在植物中的定位。[方法]根据GenBank数据库中OsWRKY17全序列设计引物,进行仉WRKY]7的RT—PCR扩增,克隆了OsWRKY17基因,将该片段与带绿色荧光蛋白(GFP)基因的质粒载体pBinGFP重组,将构建正确的表达载体pBinGFP.OsWRKY17通过农杆菌介导的花蕾浸泡法转化到拟南芥中。[结果]经菌落PCR与酶切鉴定表明成功构建了Os—WRKY17基因与GFP融合的植物表达载体pBin—GFP/OsWRKY17,并成功将OsWRKY17基因整合到拟南芥的基因组中,获得了抗性植株。【结论]OsWRKY17基因表达载体的构建为研究该基因的生理生化特性奠定了基础。
[目的]研究水稻OsWRKY17基因的生理生化特性,確定OsWRKY17蛋白在植物中的定位。[方法]根據GenBank數據庫中OsWRKY17全序列設計引物,進行仉WRKY]7的RT—PCR擴增,剋隆瞭OsWRKY17基因,將該片段與帶綠色熒光蛋白(GFP)基因的質粒載體pBinGFP重組,將構建正確的錶達載體pBinGFP.OsWRKY17通過農桿菌介導的花蕾浸泡法轉化到擬南芥中。[結果]經菌落PCR與酶切鑒定錶明成功構建瞭Os—WRKY17基因與GFP融閤的植物錶達載體pBin—GFP/OsWRKY17,併成功將OsWRKY17基因整閤到擬南芥的基因組中,穫得瞭抗性植株。【結論]OsWRKY17基因錶達載體的構建為研究該基因的生理生化特性奠定瞭基礎。
[목적]연구수도OsWRKY17기인적생리생화특성,학정OsWRKY17단백재식물중적정위。[방법]근거GenBank수거고중OsWRKY17전서렬설계인물,진행장WRKY]7적RT—PCR확증,극륭료OsWRKY17기인,장해편단여대록색형광단백(GFP)기인적질립재체pBinGFP중조,장구건정학적표체재체pBinGFP.OsWRKY17통과농간균개도적화뢰침포법전화도의남개중。[결과]경균락PCR여매절감정표명성공구건료Os—WRKY17기인여GFP융합적식물표체재체pBin—GFP/OsWRKY17,병성공장OsWRKY17기인정합도의남개적기인조중,획득료항성식주。【결론]OsWRKY17기인표체재체적구건위연구해기인적생리생화특성전정료기출。
[Objective] To study the physiological biochemical characteristic of Os- WRKY17 in rice and identify the subcellular location of OsWRKY17. [Method] The primer of the OsWRKY17 gene was designed according to the full-length sequence of OsWRKY17 in Genbank and was cloned by RT-PCR. The cloned fragment was then recombined with the green fluorescent protein gene of plasmid vector pBinGFP. The recombinant plasmid pBinGFP-OsWRKY17 was transformed into Arabidopsis through Agrobacterium tumefaciens strain GV3101. [Result] Colony PCR and diges- tion identification proved that the plant expression vector pBinGFP-OsWRKY17 was successfully constructed by the fusion of OsWRKY17 and GFP, and the expression vector was successfully transformed into the genome of Arabidopsis, there by ob- taining a resistant plant. [Conclusion] The construction of OsWRKY17 expression vector established the foundation for study on the physiological the biochemical char- acteristics of QsWRKY17.