CAJ | 학술논문

[目的]了解油藏微生物ThermoanaerobacterspDF3的生理生化特性，优化木糖产乙醇培养方案。[方法]利用厌氧分离技术从大港油田油层采出液中分离到一株产乙醇厌氧杆菌DF3采用生理生化鉴定与16SrDNA序列的系统发育学分析其系统发育地位，用气相色谱分析其代谢产物。[结果]菌株DF3是一株严格厌氧的嗜热细菌，呈直杆状，G-菌体大小为O．42μm×（1．60～5．20）μm，单生成对或成串生，产顶端芽孢；生长温度为45～78℃（最适65℃），能利用葡萄糖、木糖、果糖、核糖、甘露糖、阿拉伯糖、蔗糖、半乳糖、乳糖、纤维二糖、松三糖、棉子糖、淀粉等作为底物；其16SrRNA与zpseud。ethanolicw相似性为99．7％发酵葡萄糖与木糖的主要产物为乙醇，培养方案优化后其代谢木糖产乙醇终浓度为2．0g/L。[结论]通过试验证明菌株DF3是目前已知菌株中产乙醇活性较强的菌株之一，在65℃时代谢木糖能产生2．0g/L的乙醇目前代谢木糖高产乙醇的菌株均由国外分离获得，菌株DF3的分离获得为我国研究木质纤维素产乙醇提供了优良的出发菌株。
[목적]료해유장미생물ThermoanaerobacterspDF3적생리생화특성，우화목당산을순배양방안。[방법]이용염양분리기술종대항유전유층채출액중분리도일주산을순염양간균DF3채용생리생화감정여16SrDNA서렬적계통발육학분석기계통발육지위，용기상색보분석기대사산물。[결과]균주DF3시일주엄격염양적기열세균，정직간상，G-균체대소위O．42μm×（1．60～5．20）μm，단생성대혹성천생，산정단아포；생장온도위45～78℃（최괄65℃），능이용포도당、목당、과당、핵당、감로당、아랍백당、자당、반유당、유당、섬유이당、송삼당、면자당、정분등작위저물；기16SrRNA여zpseud。ethanolicw상사성위99．7％발효포도당여목당적주요산물위을순，배양방안우화후기대사목당산을순종농도위2．0g/L。[결론]통과시험증명균주DF3시목전이지균주중산을순활성교강적균주지일，재65℃시대사목당능산생2．0g/L적을순목전대사목당고산을순적균주균유국외분리획득，균주DF3적분리획득위아국연구목질섬유소산을순제공료우량적출발균주。
[Objective] The aim was to study the physiological and biochemical char- acteristics of Thermoanaerobacter sp DF3 in petroleum reservoirs and optimize the culture plan of producing ethanol from xylose. [Method] DF3, an anaerobic bacillus producing ethanol, was isolated from produced liquid from oil layer of Dagang oil field with anaerobic isolation technique. The phylogenetic position was analyzed by physiological and biochemical identification and phylogeny of 16S rDNA sequence. The metabolites were analyzed by gas chromatograph. [Result] The strain DF3 was a strict anaerobic thermophilic bacterium, which was straight in rod shape,and gram negative. Besides, it was 0.42 μmx（1.60-5.20） iJm in length. The strains can be soli- tary,in pairs or string and apical spore usually produced. Its growth temperature was 45-78 and 65 ℃ was the optimum. Many substances could be used as carbon sources, including glucose, xylose, fructose, ribose, mannose, arabinose, sucrose, galactose, lactose, cellobiose, melizitose, raffinose-, and starch. The similarity between strain DF3 and T. pseudoethanolicus achieved 99.7%. The main product of glucose and xylose fermentation was ethanol. After the culture plan was optimized,the final concentration of ethanol was 2.0 g/L. [Conclusion] It was proved through experiments that the strain DF3 was one of the strains with higher activity to produce ethanol at present and it could produce 2.0 g/L ethanol from xylose metabolization at 65 ℃. It has been demonstrated that DF3 is one of the known strains with high-production to produce ethanol,for example, 2.0 g/L ethano at 65℃. Presently, all the high-yield ethanol can be produced from metabolic xylose strains of metabolic xylose were isolat- ed by foreign countries, therefore, isolation of strain DF3 has provided an excellent original strain for studying ethanol production from lignocellulose in China.