CAJ | 학술논문

以发酵条件优化的菌株Penicilliumglaucum NS3为出发菌，采用紫外线、亚硝酸钠复合诱变，在UV（15W、距30cm辐照9min）、0．3％亚硝酸钠协同诱变条件下，经多轮反复诱变，得到茵株Penicillium glaucum NS3e1，其发酵液CMC酶活和滤纸酶活分别达到400．07IU／mL和9．47IU／mL，比出发茵酶活309Iu／mL和7．37IU／mL分别提高29．47％和28．35％。
이발효조건우화적균주Penicilliumglaucum NS3위출발균，채용자외선、아초산납복합유변，재UV（15W、거30cm복조9min）、0．3％아초산납협동유변조건하，경다륜반복유변，득도인주Penicillium glaucum NS3e1，기발효액CMC매활화려지매활분별체도400．07IU／mL화9．47IU／mL，비출발인매활309Iu／mL화7．37IU／mL분별제고29．47％화28．35％。
After fermetation conditions optimising of the Penicillium glaucum NS3, a several of synergistic mutation have been carry out for increasing its cellulase - producing capacity. A higher cellulase - producing strain named NS3cl had been gotten, which was breeded through inducing mutation treatmentt of ultraviolet light and sodium nitrite. The optimal dosage of mutant by UV and sodium nitrite was 9 minutes with 15W UV light in 30em distance and 0.3% concentration. Under the repeated synergistic mutations, the CMCase and FPAase produced by mutant Penicillium glaucum NS3cl reached 400.07 IU/ mL and 9.47 IU/mL, which increased 29.47% and 28.35% than that of the original activities 309 IU/mL and 7.37 IU/mL.